首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝血因子XIII A1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F13A1含量。F13A1是一个重要靶点。它参与凝血过程，F13A1编码的凝血因子XIII A亚基在稳定纤维蛋白凝块中起关键作用。科学家围绕其功能和作用机制开展研究，探索通过调节它来干预凝血异常相关疾病的新策略。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研领域中对凝血机制的基础研究、药物开发中凝血调节剂的体外活性评估，或心血管疾病模型动物中凝血相关生物标志物的动态分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F13a1 ELISA kit; F13aCoagulation factor XIII A chain ELISA kit; Coagulation factor XIIIa ELISA kit; EC 2.3.2.13 ELISA kit; Protein-glutamine gamma-glutamyltransferase A chain ELISA kit; Transglutaminase A chain ELISA kit
缩写：F13A1
Uniprot No.：Q8BH61
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse F13A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:800 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of mouse F13A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
| EDTA plasma (n=4) | 98 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.6842.6572.6712.487502.3982.3172.3582.174251.8651.8981.8821.69812.51.2471.2141.2311.0476.250.6730.6990.6860.5023.120.4880.4980.4930.3091.560.3570.3860.3720.18800.1830.1840.184 | ||||||
货期：3-5 working days
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