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产品描述：人凝血因子Ⅴ(FⅤ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F5含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于科研领域中对凝血机制的基础研究、抗凝药物开发筛选或病理模型中FⅤ水平动态变化的分析。为研究FⅤ在止血与血栓形成中的调控作用、评估实验动物模型或细胞模型的凝血功能提供了可靠工具，尤其适用于心血管疾病、遗传性凝血障碍及相关药物作用机制的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Activated protein C cofactor ELISA Kit; APC cofactor ELISA Kit; coagulation factor V (proaccelerin; labile factor) ELISA Kit; Coagulation factor V ELISA Kit; coagulation factor V jinjiang A2 domain ELISA Kit; Coagulation factor V light chain ELISA Kit; F5 ELISA Kit; FA5_HUMAN ELISA Kit; Factor V Leiden ELISA Kit; FactorV ELISA Kit; FVL ELISA Kit; Labile factor ELISA Kit; PCCF ELISA Kit; Proaccelerin ELISA Kit; proaccelerin; labile factor ELISA Kit; Protein C cofactor ELISA Kit; RPRGL1 ELISA Kit; THPH2 ELISA Kit
缩写：F5
Uniprot No.：P12259
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FⅤ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:2000 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:4000 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:8000 | Average % | 103 | |||||
| Range % | 99-107 | ||||||
回收率：
| The recovery of human FⅤ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 93 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2002.4442.5362.4902.3601002.1862.0992.1432.013501.7541.6821.7181.588251.1561.1041.1301.00012.50.6680.7150.6920.5626.250.4030.3870.3950.2653.120.2890.2840.2870.15700.1320.1280.130 | |||||||
货期：3-5 working days
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