首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝血因子Ⅴ(FⅤ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的F5含量。F5即凝血因子Ⅴ，作为凝血过程关键因子，在凝血共同途径中起加速凝血酶原向凝血酶转化作用。其基因突变会导致易栓症或出血性疾病等。研究聚焦其结构功能、与疾病关联机制，为相关疾病诊断治疗提供理论支撑。试剂盒检测范围为78 ng/mL-5000 ng/mL，适用于基础科研中探究凝血机制、评估药物或基因干预对凝血功能的影响，以及构建凝血相关疾病模型（如遗传性凝血缺陷或血栓性疾病）时监测FⅤ的表达水平变化。为血液学、药理学及心血管疾病研究提供可靠工具，实验结果仅用于科研分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F5Coagulation factor V ELISA Kit; Activated protein C cofactor) [Cleaved into: Coagulation factor V heavy chain; Coagulation factor V light chain] ELISA Kit
缩写：FⅤ
Uniprot No.：O88783
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅤ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse FⅤ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 88 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected50002.3512.5212.4362.24025001.8231.9071.8651.66912501.3541.2941.3241.1286250.8530.8930.8730.6773120.6120.6070.6100.4141560.4010.4210.4110.215780.2980.2870.2930.09700.2000.1920.196 | ||||||
货期：3-5 working days
×
