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产品描述：兔凝血因子VII(F7)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F7含量。试剂盒检测范围为3.9 ng/mL-250 ng/mL，适用于科研领域对凝血机制、出血性疾病模型或药物干预效果的研究；支持研究者高效完成心血管疾病模型、肝脏功能评估或抗凝药物开发等实验中的F7水平监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
缩写：F7
Uniprot No.：P98139
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：3.9 ng/mL-250 ng/mL
灵敏度：1 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:40 | Average % | 104 | |||||
| Range % | 100-107 | ||||||
| 1:80 | Average % | 87 | |||||
| Range % | 83-90 | ||||||
| 1:160 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
回收率：
| The recovery of rabbit F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-93 | |||||
| EDTA plasma (n=4) | 110 | 107-113 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 2500.1340.1300.132 1250.2190.2210.220 62.50.3780.3810.380 31.20.5650.5810.573 15.60.8680.8580.863 7.81.2091.2361.223 3.91.5981.5691.584 02.3992.4012.400 | |||||||
货期：3-5 working days
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