首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝血因子Ⅷ（FⅧ）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的F8含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研场景下对小鼠凝血功能的机制研究，例如血友病A模型小鼠的病理分析、药物治疗后凝血功能恢复评估，或基因编辑干预效果的定量追踪。为凝血相关基础研究及药物开发提供稳定可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F8 ELISA kit; Cf8 ELISA kit; F8cCoagulation factor VIII ELISA kit; Procoagulant component ELISA kit
缩写：F8
Uniprot No.：Q06194
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅧ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:2000 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:4000 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of mouse FⅧ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 80-92 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5642.4872.5262.381102.1172.2542.1862.04151.5751.6671.6211.4762.51.0651.0741.0700.9251.250.6050.6220.6140.4690.6250.3320.3440.3380.1930.3120.2380.2320.2350.09000.1460.1440.145 | ||||||
货期：3-5 working days
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