首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠凝血因子Ⅺ(FⅪ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F11含量。试剂盒检测范围为0.47 ng/mL-30 ng/mL，该产品可应用于凝血机制研究、血栓性疾病动物模型分析、抗凝药物开发等科研领域，尤其适用于心血管疾病模型小鼠的血液样本检测，以及肝、肾等组织中FⅪ表达水平的比较研究，为探索凝血因子调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F11Coagulation factor XI ELISA kit; FXI ELISA kit; EC 3.4.21.27 ELISA kit; Plasma thromboplastin antecedent ELISA kit; PTA) [Cleaved into: Coagulation factor XIa heavy chain; Coagulation factor XIa light chain] ELISA kit
缩写：F
Uniprot No.：Q91Y47
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.47 ng/mL-30 ng/mL
灵敏度：0.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅪ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of mouse FⅪ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 98 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 300.1470.1390.143 150.2190.2250.222 7.50.3450.3260.336 3.750.5130.5360.525 1.8750.8570.8620.860 0.941.1791.1981.189 0.471.5381.5591.549 02.5332.5422.538 | ||||||
货期：3-5 working days
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