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产品描述：猪肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的FABP2含量。FABP2是一个靶点。它在脂肪酸摄取、转运等过程中发挥重要作用。研究聚焦于其与代谢性疾病关联机制，如肥胖、糖尿病等。解析其结构和功能，有助于深入了解疾病发病机制，为开发针对性治疗药物提供理论支持。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于肠道炎症、缺血再灌注损伤等病理机制的科研分析；适用于科研场景中动物模型肠道损伤的动态监测、营养干预对肠道功能影响评估，或药物研发中肠道保护效果的量化分析；为消化道相关疾病的基础研究提供标准化检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FABP2 ELISA Kit; FABPIFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
缩写：FABP2
Uniprot No.：Q45KW7
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-93 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of pig iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 81-89 | ||||
| EDTA plasma (n=4) | 99 | 93-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.1292.1382.1341.97625001.6281.6561.6421.48412501.0841.0871.0860.9286250.6070.6380.6230.4653120.4450.4260.4360.2781560.3480.3230.3360.178780.2680.2510.2600.10200.1570.1590.158 | ||||||
货期：3-5 working days
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