首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的FABP2含量。FABP2 即脂肪酸结合蛋白 2，主要在小肠表达。它可结合脂肪酸并参与细胞内脂质代谢。研究机制方面，其结构使其能特异性结合脂肪酸，转运至代谢位点，与肥胖、糖尿病等代谢疾病发病机制密切相关，是潜在治疗靶点。试剂盒检测范围为1.25 ng/mL-80 ng/mL，被广泛用于肠道屏障功能障碍、缺血再灌注损伤及炎症性肠病等研究的生物标志物；可灵活应用于动物模型肠道损伤动态监测、药物干预效果评估或疾病机制研究；适用于实验室常规 ELISA 检测系统，为消化道相关科研提供稳定可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fabp2 ELISA kit; FabpiFatty acid-binding protein ELISA kit; intestinal ELISA kit; Fatty acid-binding protein 2 ELISA kit; Intestinal-type fatty acid-binding protein ELISA kit; I-FABP ELISA kit
缩写：FABP2
Uniprot No.：P02693
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 98-108 | |||||
回收率：
| The recovery of rat iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 93 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.6952.6712.6832.536401.9151.9011.9081.761201.3061.3341.3201.173100.8840.8990.8920.74550.5170.5370.5270.3802.50.3750.3840.3800.2331.250.2110.2330.2220.07500.1450.1490.147 | ||||||
货期：3-5 working days
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