首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：大鼠脂肪酸结合蛋白4，脂肪细胞型(FABP4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的FABP4含量。FABP4 是一种脂肪酸结合蛋白。它在脂质代谢和炎症反应等生理过程中发挥重要作用。研究机制上，FABP4 参与脂肪酸的摄取、转运和代谢调节，其异常表达与糖尿病、肥胖症、心血管疾病等病理状态密切相关，是潜在治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，可满足细胞能量代谢研究、脂肪组织功能评估以及代谢性疾病机制探索等科研需求，例如在糖尿病模型大鼠中动态监测脂肪因子表达水平，或体外药物干预实验中评估化合物对脂肪细胞代谢的影响。支持科研人员在体外实验模型中系统研究FABP4的生物学功能及其在代谢调节网络中的作用机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fabp4Fatty acid-binding protein ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; ALBP ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; A-FABP ELISA Kit; AFABP ELISA Kit; Fatty acid-binding protein 4 ELISA Kit
缩写：FABP4
Uniprot No.：P70623
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-100 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-99 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 90 | 82-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1001.9741.9131.9441.817 501.7211.6811.7011.574 251.4161.3111.3641.237 12.50.9580.8970.9280.801 6.250.6690.6290.6490.522 3.120.3950.3640.3800.253 1.560.2170.2070.2120.085 00.1310.1230.127 | ||||||
货期：3-5 working days
×
