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产品描述：人FAM132B蛋白(FAM132B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的FAM132B含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞模型的功能研究、动物实验样本的分子机制探索，以及临床前研究中生物标志物的定量分析等科研场景，为代谢调控通路、细胞分化机制等基础研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; ERFE_HUMAN ELISA Kit; Erythroferrone ELISA Kit; FAM132B ELISA Kit; Myonectin ELISA Kit
缩写：FAM132B
Uniprot No.：Q4G0M1
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of human FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-102 | ||||
| EDTA plasma (n=4) | 88 | 84-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4552.4302.4432.3445002.2012.2502.2262.1272501.7051.8541.7801.6811251.1101.2021.1561.05762.50.6990.7410.7200.62131.20.4350.4220.4290.33015.60.2660.2700.2680.16900.1030.0950.099 | ||||||
货期：3-5 working days
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