| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RANBP1 |
| Uniprot No | P43487 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RANBP1重组蛋白的3篇参考文献示例(注：文献信息为虚构示例，仅供格式参考)：
1. **标题**：*Recombinant RANBP1 Expression and Its Role in Nuclear Transport*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌系统表达重组RANBP1蛋白，并验证其促进RanGTP水解的功能，阐明了其在核质运输中对Ran蛋白循环的调控机制。
2. **标题**：*Structural Analysis of RANBP1 in Mitotic Regulation*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析重组RANBP1的三维结构，揭示其与RanGTP的结合位点，并发现其异常表达导致有丝分裂纺锤体组装缺陷。
3. **标题**：*RANBP1 Interaction with Importin-β in Cancer Cell Metastasis*
**作者**：Wang Y, et al.
**摘要**：利用重组RANBP1蛋白进行体外结合实验，证实其通过与Importin-β相互作用调控肿瘤细胞中蛋白转运，影响癌细胞迁移和侵袭能力。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“RANBP1 recombinant protein”获取。
**Background of RANBP1 Recombinant Protein**
RANBP1 (Ran-binding protein 1) is a cytoplasmic protein that plays a critical role in regulating nucleocytoplasmic transport, mitotic progression, and microtubule dynamics. It functions as a key cofactor for Ran GTPase, a small GTPase essential for maintaining the asymmetry of Ran-GTP/Ran-GDP gradients across the nuclear envelope. This gradient drives the directional transport of proteins and RNA molecules between the nucleus and cytoplasm through interactions with importins and exportins. RANBP1 binds preferentially to Ran-GTP, promoting the dissociation of cargo complexes in the cytoplasm and facilitating the recycling of transport receptors.
Structurally, RANBP1 contains a conserved Ran-binding domain (RanBD) that mediates its interaction with Ran. It also participates in mitotic processes by associating with the RANBP1-RanGAP1-SUMO1 complex, which stabilizes kinetochore-microtubule attachments and ensures proper chromosome segregation. Dysregulation of RANBP1 has been linked to genomic instability, cancer progression, and neurodegenerative disorders, highlighting its importance in cellular homeostasis.
Recombinant RANBP1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and bioactivity. It serves as a vital tool for *in vitro* studies, including assays investigating nuclear transport mechanisms, Ran GTPase cycle dynamics, and mitotic regulation. Its applications extend to drug discovery, particularly in targeting pathways implicated in cancer and diseases associated with transport defects. By enabling precise manipulation of RANBP1-Ran interactions, recombinant RANBP1 has advanced our understanding of cellular trafficking and mitosis, offering potential therapeutic avenues for related pathologies.
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