| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAMP3 |
| Uniprot No | O60896 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-118aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MRAGGCNETG MLERLPLCGK AFADMMGKVD VWKWCNLSEF IVYYESFTNC TEMEANVVGC YWPNPLAQGF ITGIHRQFFS NCTVDRVHLE DPPDEV |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于RAMP3重组蛋白的模拟参考文献(基于典型研究主题，非真实文献)：
1. **文献名称**: "Recombinant RAMP3 Enhances Adrenomedullin Receptor Signaling in Vascular Smooth Muscle Cells"
**作者**: Smith A.B., et al.
**摘要**: 研究利用重组RAMP3蛋白与CLR受体共表达，证明其可增强肾上腺髓质素(AM)介导的血管平滑肌细胞cAMP信号通路，揭示RAMP3在调节血管舒张中的关键作用。
2. **文献名称**: "Structural Characterization of RAMP3-Calcitonin Receptor Complex by Cryo-EM"
**作者**: Tanaka K., et al.
**摘要**: 通过冷冻电镜解析重组RAMP3与降钙素受体的复合物结构，阐明RAMP3如何通过跨膜结构域调控受体配体选择性，为靶向药物设计提供依据。
3. **文献名称**: "RAMP3 Recombinant Protein Attenuates Tumor Angiogenesis in Mouse Models"
**作者**: Chen L., et al.
**摘要**: 在小鼠肿瘤模型中，外源性重组RAMP3蛋白通过抑制VEGF信号通路减少肿瘤血管生成，提示其潜在抗肿瘤治疗价值。
注：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以关键词"RAMP3 recombinant"检索真实文献。
**Background of RAMP3 Recombinant Protein**
Receptor Activity-Modifying Protein 3 (RAMP3) is a member of the RAMP family, a group of single-transmembrane proteins that regulate the trafficking and ligand specificity of G protein-coupled receptors (GPCRs), particularly the calcitonin receptor-like receptor (CRLR). RAMP3. along with RAMP1 and RAMP2. modulates CRLR’s function, enabling its interaction with distinct ligands: RAMP3-CRLR complexes primarily recognize adrenomedullin (AM), a peptide involved in cardiovascular homeostasis, angiogenesis, and inflammation.
Structurally, RAMP3 contains an extracellular N-terminal domain, a transmembrane helix, and a short cytoplasmic tail. Its interaction with CRLR stabilizes the receptor’s cell surface expression and determines ligand-binding selectivity. Beyond receptor modulation, RAMP3 is implicated in intracellular signaling pathways, including cAMP and MAPK cascades, influencing cell proliferation, migration, and apoptosis.
RAMP3 is broadly expressed in tissues such as the heart, lungs, kidneys, and blood vessels. Dysregulation of RAMP3 has been linked to pathologies, including hypertension, heart failure, cancer, and metabolic disorders. For instance, RAMP3 overexpression in tumors may promote metastasis by enhancing AM-mediated signaling, while its deficiency in cardiovascular models exacerbates stress-induced cardiac dysfunction.
Recombinant RAMP3 proteins are engineered in vitro (e.g., via *E. coli* or mammalian systems) for functional studies. These proteins retain key domains for receptor interaction and are often tagged (e.g., His-tag) for purification and detection. Researchers use recombinant RAMP3 to dissect CRLR-AM signaling mechanisms, screen therapeutic agents, or explore its role in disease models. Its versatility makes it a valuable tool for probing GPCR biology and developing targeted therapies.
In summary, RAMP3 is a multifunctional chaperone protein with critical roles in receptor biology and disease, and recombinant forms enable detailed mechanistic and translational research.
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