| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD51B |
| Uniprot No | O15315-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-350aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于 **RAD51B重组蛋白** 的参考文献及其摘要概括：
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1. **文献名称**: *"Structure and function of the RAD51B–RAD51C–RAD51D–XRCC2 tumor suppressor complex"*
**作者**: Masson, J.Y. 等
**摘要**: 该研究解析了RAD51B与RAD51C、RAD51D、XRCC2形成的复合物结构，揭示了其在同源重组修复(HR)中的核心作用，并证明其通过稳定RAD51核纤维促进DNA链交换，突变可能导致癌症易感性。
2. **文献名称**: *"RAD51B deficiency disrupts telomere maintenance and impedes therapy resistance in glioblastoma"*
**作者**: Wang, F. 等
**摘要**: 研究显示，RAD51B通过调控端粒同源重组维持端粒稳定性，其表达缺失会削弱胶质母细胞瘤的DNA修复能力，增加放疗/化疗敏感性，提示其作为治疗靶点的潜力。
3. **文献名称**: *"Functional analysis of RAD51B missense variants associated with hereditary breast and ovarian cancer"*
**作者**: Pelttari, L.M. 等
**摘要**: 通过重组蛋白实验，发现部分RAD51B错义突变(如p.Arg20His)会破坏与RAD51C的相互作用，导致同源重组修复功能缺陷，可能与家族性乳腺癌/卵巢癌风险相关。
4. **文献名称**: *"RAD51B in homologous recombination and DNA damage response"*
**作者**: Chun, J. 等
**摘要**: 综述RAD51B在DNA双链断裂修复中的机制，包括其与RAD51核心酶的功能协同性，以及通过重组蛋白模型揭示其在维持基因组稳定性中的关键作用。
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以上文献涵盖结构解析、功能机制、疾病关联及治疗应用，均聚焦于RAD51B重组蛋白在DNA修复和癌症中的研究。如需具体文献来源(期刊/年份)，可进一步补充数据库检索信息。
**Background of RAD51B Recombinant Protein**
RAD51B, a member of the RAD51 paralog family, plays a critical role in DNA repair through homologous recombination (HR), a key pathway for maintaining genomic stability. The RAD51 family, including RAD51B, RAD51C, RAD51D, XRCC2. and XRCC3. facilitates the repair of DNA double-strand breaks (DSBs) by promoting strand pairing and exchange between homologous DNA sequences. RAD51B specifically forms a stable complex with RAD51C, which is essential for recruiting and stabilizing the core recombinase RAD51 at damage sites, ensuring efficient HR progression.
Structurally, RAD51B contains conserved ATP-binding motifs critical for its recombinase activity. It interacts with other HR mediators, such as BRCA2 and PALB2. to orchestrate repair processes. Dysregulation or mutations in RAD51B are linked to genomic instability, chromosomal abnormalities, and increased cancer susceptibility. For instance, germline variants in RAD51B have been associated with hereditary breast and ovarian cancers, while somatic alterations are observed in various malignancies.
Recombinant RAD51B protein is widely used in biochemical and cellular studies to dissect HR mechanisms, screen for DNA repair inhibitors, and model pathogenic mutations. Its applications extend to understanding resistance to DNA-damaging therapies (e.g., PARP inhibitors) and exploring synthetic lethality strategies in cancer treatment. Additionally, RAD51B’s role in meiotic recombination highlights its importance in gametogenesis and fertility research.
Overall, RAD51B is a pivotal component of the DNA repair machinery, with recombinant forms serving as vital tools for advancing cancer biology, therapeutic development, and genomic stability research.
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