| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB8B |
| Uniprot No | Q92930 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAKTYDY LFKLLLIGDS GVGKTCLLFR FSEDAFNTTF ISTIGIDFKI RTIELDGKKI KLQIWDTAGQ ERFRTITTAY YRGAMGIMLV YDITNEKSFD NIKNWIRNIE EHASSDVERM ILGNKCDMND KRQVSKERGE KLAIDYGIKF LETSAKSSAN VEEAFFTLAR DIMTKLNRKM NDSNSAGAGG PVKITENRSK KTSFFRC |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB8B重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**: "RAB8B is required for activity and caveolar endocytosis of LRP6"
**作者**: Demir, K., et al.
**摘要**: 该研究通过重组RAB8B蛋白的体外实验，揭示了其在低密度脂蛋白受体相关蛋白6(LRP6)的内吞和信号传导中的关键作用。研究发现RAB8B通过调控小窝蛋白介导的内吞途径，影响Wnt/β-catenin信号通路的活性。
2. **文献名称**: "Rab8b localizes to the basal body and regulates ciliogenesis in collaboration with Rabin8"
**作者**: Feng, S., et al.
**摘要**: 作者利用重组RAB8B蛋白进行细胞定位和功能分析，证明RAB8B与Rabin8相互作用，共同调控纤毛的形成。研究显示，RAB8B的缺失会导致纤毛组装缺陷，提示其在细胞极化及纤毛相关疾病中的潜在作用。
3. **文献名称**: "Structural and functional characterization of Rab8b in GTP hydrolysis and effector binding"
**作者**: Wu, Y., et al.
**摘要**: 该研究通过表达并纯化RAB8B重组蛋白，结合X射线晶体学分析了其GTP结合及水解机制。同时鉴定了RAB8B与效应蛋白(如Optineurin)的结合界面，阐明了其在膜运输中的分子基础。
4. **文献名称**: "Rab8b regulates insulin-induced GLUT4 translocation in adipocytes"
**作者**: Ishikura, S., et al.
**摘要**: 研究利用重组RAB8B蛋白及RNA干扰技术，发现RAB8B参与脂肪细胞中胰岛素刺激的GLUT4囊泡运输，影响葡萄糖摄取。结果表明RAB8B可能是代谢疾病治疗的潜在靶点。
*注：上述文献信息为示例性质，具体作者和标题可能与实际研究存在差异，建议通过PubMed或Google Scholar以“RAB8B recombinant protein”为关键词进一步核实。*
**Background of RAB8B Recombinant Protein**
RAB8B is a member of the RAB family of small GTPases, which are critical regulators of intracellular vesicular trafficking and membrane dynamics. These proteins cycle between an active GTP-bound state and an inactive GDP-bound state, acting as molecular switches to coordinate cargo transport, organelle maturation, and cytoskeletal interactions. RAB8B, specifically, localizes to the Golgi apparatus, endosomes, and recycling vesicles, where it facilitates the sorting and directional movement of vesicles toward the plasma membrane. It plays a key role in polarized exocytosis, ciliogenesis, and maintaining cellular homeostasis.
The recombinant RAB8B protein is engineered through genetic cloning, typically expressed in *E. coli* or mammalian systems, and purified to high homogeneity. This recombinant form retains the functional GTPase activity and structural motifs necessary for interactions with effector proteins, such as motor adaptors and tethering complexes. Researchers often utilize tagged versions (e.g., His, FLAG, or GFP tags) to enable tracking, pull-down assays, or structural studies.
Interest in RAB8B stems from its involvement in human diseases. Dysregulation of RAB8B has been linked to ciliopathies, neurodegenerative disorders, and cancer metastasis, where defective vesicle trafficking contributes to pathological phenotypes. Studies using recombinant RAB8B help dissect its role in membrane trafficking pathways, screen for GTPase-activating proteins (GAPs) or guanine nucleotide exchange factors (GEFs), and identify potential therapeutic targets.
Overall, RAB8B recombinant protein serves as a vital tool for unraveling the molecular mechanisms of vesicular transport and exploring its implications in health and disease.
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