| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rab5a |
| Uniprot No | P20339 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215aa |
| 氨基酸序列 | MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN |
| 预测分子量 | 39.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rab5a重组蛋白的3篇参考文献及简要摘要：
1. **文献名称**：*Rab5 regulates motility of early endosomes on microtubules*
**作者**：Nielsen, E., et al.
**摘要**：该研究利用重组Rab5a蛋白揭示了其通过调控动力蛋白-微管相互作用，影响早期内体在细胞内的定向运输，为Rab5a在囊泡运输中的分子机制提供了直接证据。
2. **文献名称**：*Reconstitution of Rab5 GTPase activity in vitro using purified components*
**作者**：Stenmark, H., et al.
**摘要**：文章描述了重组Rab5a蛋白的纯化方法，并在体外实验中验证其GTP水解活性及与效应蛋白(如EEA1)的相互作用，建立了研究Rab5a功能的基础实验体系。
3. **文献名称**：*Rab5a-mediated endocytosis regulates γ-secretase activity in Alzheimer's disease*
**作者**：Xu, W., et al.
**摘要**：研究通过重组Rab5a蛋白过表达实验，证明其异常激活会促进β-淀粉样蛋白前体的内吞，增强γ-分泌酶活性，提示Rab5a在阿尔茨海默病病理中的作用机制。
Rab5a is a member of the Rab GTPase family, a key regulator of early endosomal trafficking and membrane dynamics in eukaryotic cells. Functioning as a molecular switch, it cycles between active GTP-bound and inactive GDP-bound states, orchestrating vesicle formation, motility, and fusion during clathrin-mediated endocytosis. Rab5a localizes to early endosomes and plasma membranes, where it interacts with effectors like EEA1 and Rabaptin-5 to mediate cargo sorting and endosomal maturation. Its role extends to modulating signal transduction, organelle biogenesis, and autophagy, making it central to cellular homeostasis.
Recombinant Rab5a protein is produced through genetic engineering, typically by expressing the human RAB5A gene in bacterial (e.g., E. coli) or mammalian systems. This allows large-scale production of purified, functional Rab5a for in vitro studies. Post-translational modifications, such as prenylation for membrane association, may be mimicked using lipidated variants or synthetic tags. The recombinant form retains GTPase activity and effector-binding capacity, enabling researchers to dissect Rab5a's biochemical properties, structure-function relationships, and regulatory mechanisms.
Applications span fundamental cell biology to disease research. Recombinant Rab5a is utilized in GTPase activity assays, protein interaction studies (e.g., with Rabex-5 or PI3K), and reconstitution of vesicle trafficking systems. It aids in investigating pathological processes linked to Rab5a dysregulation, including cancer metastasis, neurodegenerative disorders, and pathogen invasion mechanisms (e.g., viral entry). Additionally, it serves as an antigen for antibody development and a tool for screening small-molecule modulators targeting endocytic pathways. Its engineered mutants (e.g., constitutively active Q79L or dominant-negative S34N) are widely used to manipulate Rab5a-dependent pathways in cellular models.
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