| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB3D |
| Uniprot No | O95716 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMASAGDTQAGPRDAADQNFDYMFKLLLIGN SSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAG QERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQV ILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLV DVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RAB3D重组蛋白的相关文献摘要信息(虚拟文献示例，实际需通过数据库检索验证)：
1. **文献名称**: "Expression and Functional Analysis of Recombinant RAB3D in Pancreatic Acinar Cells"
**作者**: Smith J. et al.
**摘要**: 研究在大肠杆菌中重组表达RAB3D蛋白，发现其通过调控囊泡运输影响胰腺腺泡细胞的酶分泌功能，证实其GTPase活性对胞吐过程的关键作用。
2. **文献名称**: "RAB3D Regulates Mucin Secretion in Airway Epithelial Cells via Recombinant Protein Interaction"
**作者**: Lee H. & Zhang Y.
**摘要**: 利用重组RAB3D进行体外实验，揭示其通过结合SNARE复合体调控呼吸道杯状细胞的黏液分泌，为慢性阻塞性肺病治疗提供潜在靶点。
3. **文献名称**: "Cryo-EM Structure of Recombinant RAB3D-GTP Complex Reveals Membrane Trafficking Mechanism"
**作者**: Garcia R. et al.
**摘要**: 首次解析重组人源RAB3D蛋白与GTP结合的冷冻电镜结构，阐明其效应蛋白结合域构象变化，为靶向囊泡运输的药物设计提供结构基础。
提示：实际文献建议通过PubMed/Google Scholar以关键词"RAB3D recombinant protein"或结合具体研究领域(如"secretion"/"vesicle trafficking")检索近5-10年高被引论文。
**Background of RAB3D Recombinant Protein**
RAB3D, a member of the RAB GTPase family, plays a critical role in regulating intracellular membrane trafficking and vesicle-mediated secretion. It is primarily associated with secretory granules in specialized cell types, including pancreatic acinar cells, adipocytes, and gastric parietal cells. As a small GTP-binding protein, RAB3D cycles between GTP-bound (active) and GDP-bound (inactive) states, a process tightly controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). This cycling enables RAB3D to coordinate vesicle docking, fusion, and release of cargo, particularly in exocytosis pathways.
The recombinant RAB3D protein is engineered using genetic cloning techniques, often expressed in bacterial or mammalian systems to ensure proper post-translational modifications, such as prenylation, which is essential for membrane association. Purified recombinant RAB3D retains functional GTPase activity and interacts with effector molecules like RAB3D-interacting proteins (e.g., synaptotagmin, rabphilin), making it invaluable for studying vesicle dynamics, secretory mechanisms, and membrane trafficking disorders.
Research on RAB3D has highlighted its dual roles in health and disease. While it supports normal secretory functions, dysregulation of RAB3D is implicated in pathologies such as diabetes, obesity, and certain cancers. For instance, reduced RAB3D expression in pancreatic β-cells impairs insulin granule exocytosis, contributing to diabetes pathogenesis. Conversely, its overexpression in tumors may promote invasion or metastasis.
Recombinant RAB3D serves as a vital tool for structural studies, drug screening, and functional assays, enabling insights into GTPase signaling networks and therapeutic targeting of trafficking-related diseases. Its application spans cell biology, biochemistry, and translational research, underscoring its significance in both basic and clinical contexts.
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