| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB3B |
| Uniprot No | P20337 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MASVTDGKTG VKDASDQNFD YMFKLLIIGN SSVGKTSFLF RYADDTFTPA FVSTVGIDFK VKTVYRHEKR VKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWATQI KTYSWDNAQV ILVGNKCDME EERVVPTEKG QLLAEQLGFD FFEASAKENI SVRQAFERLV DAICDKMSDS LDTDPSMLGS SKNTRLSDTP PLLQQNCSC |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于RAB3B重组蛋白的参考文献摘要概括：
1. **文献名称**: "Expression and Purification of Functional Recombinant RAB3B in Escherichia coli for GTPase Activity Studies"
**作者**: Tanaka et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达可溶性RAB3B重组蛋白的优化方法，通过His标签纯化获得高纯度蛋白。功能实验表明重组RAB3B具有GTP水解活性，并验证了其与效应蛋白RIM2的相互作用。
2. **文献名称**: "Structural Insights into RAB3B-Lipid Interactions Using Recombinant Protein Crystallography"
**作者**: Müller et al.
**摘要**: 利用重组RAB3B蛋白的晶体结构分析，揭示了其C端脂质修饰(香叶酰化)对膜结合的关键作用，并发现其Switch II结构域在GDP/GTP转换中的构象变化，为囊泡运输机制提供结构依据。
3. **文献名称**: "RAB3B Regulates Exocytosis in Neuroendocrine Cells via Recombinant Protein Reconstitution Assays"
**作者**: Zhang & Chen
**摘要**: 通过重组RAB3B蛋白与合成脂质体的体外重建实验，证明RAB3B通过招募效应蛋白Munc18-1调控囊泡锚定过程，并发现其磷酸化修饰(Ser123)显著抑制分泌功能。
*注：以上文献信息为示例性概括，实际研究中建议通过PubMed或Web of Science以“RAB3B recombinant protein”为关键词检索最新文献。若需具体论文，可提供数据库访问权限进一步筛选。*
**Background of RAB3B Recombinant Protein**
RAB3B is a member of the RAB family of small GTPases, which are critical regulators of intracellular vesicle trafficking and exocytosis. These proteins cycle between active GTP-bound and inactive GDP-bound states, acting as molecular switches to control membrane fusion, cargo sorting, and vesicle docking. RAB3B, specifically, is implicated in secretory pathways, particularly in neurotransmitter release from synaptic vesicles and hormone secretion from endocrine cells. It localizes to secretory vesicles and interacts with effector proteins like RAB3-interacting molecules (RIMs) and synaptotagmin to modulate vesicle priming and fusion.
Recombinant RAB3B protein is engineered for in vitro studies to dissect its biochemical properties, structure-function relationships, and interactions. Produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells), the recombinant protein retains key functional domains, including the GTP-binding region and hypervariable C-terminal domain responsible for membrane association. Post-translational modifications (e.g., prenylation) may be added to enhance biological activity.
Research on RAB3B has linked it to neurological disorders, endocrine dysregulation, and cancer. For example, aberrant RAB3B expression is observed in certain tumors, where it may influence cell proliferation, metastasis, or drug resistance. Recombinant RAB3B enables investigations into its role in these pathologies, serving as a tool for binding assays, enzymatic activity studies, or screening therapeutic compounds. Additionally, it aids in structural studies (e.g., crystallography) to map interaction interfaces or design targeted inhibitors.
Overall, RAB3B recombinant protein provides a versatile platform for exploring vesicle trafficking mechanisms and developing disease-related interventions.
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