| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB34 |
| Uniprot No | Q9BZG1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-259aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMNILAPVRRDRVLAELPQCLRKEAALHGHK DFHPRVTCACQEHRTGTVGFKISKVIVVGDLSVGKTCLINRFCKDTFDKN YKATIGVDFEMERFEVLGIPFSLQLWDTAGQERFKCIASTYYRGAQAIII VFNLNDVASLEHTKQWLADALKENDPSSVLLFLVGSKKDLSTPAQYALME KDALQVAQEMKAEYWAVSSLTGENVREFFFRVAALTFEANVLAELEKSGA RRIGDVVRINSDDSNLYLTASKKKPTCCP |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB34重组蛋白的3篇代表性文献及其摘要概括(文献信息为示例，实际引用请核对原文)：
---
1. **文献名称**：*RAB34 regulates lysosome positioning through interaction with the effector protein*
**作者**：Wang et al. (2020)
**摘要**：本研究利用重组RAB34蛋白进行体外结合实验，发现其与溶酶体定位相关效应蛋白相互作用，揭示RAB34通过调控溶酶体空间分布影响细胞分泌功能，为细胞器运输机制提供新见解。
---
2. **文献名称**：*RAB34 overexpression promotes glioblastoma invasion via modulating vesicular trafficking*
**作者**：Smith et al. (2018)
**摘要**：通过重组RAB34蛋白的功能实验，作者发现其在胶质母细胞瘤中异常高表达，并通过调控囊泡运输促进肿瘤细胞侵袭，提示RAB34可能成为癌症治疗的潜在靶点。
---
3. **文献名称**：*Structural insights into GTP binding of RAB34 through recombinant protein crystallography*
**作者**：Johnson & Lee (2019)
**摘要**：利用重组RAB34蛋白进行X射线晶体学研究，解析其与GTP结合的构象变化，阐明其GTP酶活性调控机制，为设计靶向RAB34的小分子抑制剂奠定结构基础。
---
**提示**：以上文献为示例，具体研究需查阅PubMed或Web of Science等数据库获取原文，并注意核对作者、年份及期刊信息的准确性。
**Background of RAB34 Recombinant Protein**
RAB34 is a member of the RAB family of small GTPases, which are critical regulators of intracellular membrane trafficking and vesicular transport. These proteins cycle between active GTP-bound and inactive GDP-bound states, acting as molecular switches to control organelle dynamics, cargo sorting, and vesicle fusion. RAB34. in particular, is associated with the regulation of Golgi apparatus positioning, lysosomal distribution, and ciliogenesis. It plays a role in maintaining cellular homeostasis by influencing autophagy, endosomal sorting, and secretory pathways. Dysregulation of RAB34 has been linked to pathological conditions, including cancer, neurodegenerative disorders, and ciliopathies, highlighting its importance in cellular physiology.
Recombinant RAB34 protein is produced through genetic engineering, typically using bacterial, insect, or mammalian expression systems. This involves cloning the RAB34 gene into a plasmid, expressing it in host cells, and purifying the protein via affinity chromatography. The recombinant form retains the functional GTPase activity and structural features of native RAB34. enabling researchers to study its biochemical properties, interactions with effector proteins, and regulatory mechanisms *in vitro*.
Studies using recombinant RAB34 have advanced our understanding of its role in membrane trafficking and disease pathways. For instance, it has been implicated in tumor cell invasion by promoting matrix metalloproteinase secretion and in ciliary dysfunction by disrupting primary cilium formation. Additionally, recombinant RAB34 serves as a tool for screening therapeutic compounds targeting GTPase activity or protein-protein interactions. Its application spans basic research, drug discovery, and diagnostic development, underscoring its versatility as a molecular tool in both cellular and biomedical studies.
×
