| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB31 |
| Uniprot No | Q13636 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-195aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHLHKFLIWDTA GQERFHSLAP MYYRGSAAAV IVYDITKQDS FYTLKKWVKE LKEHGPENIV MAIAGNKCDL SDIREVPLKD AKEYAESIGA IVVETSAKNA INIEELFQGI SRQIPPLDPH ENGNNGTIKV EKPTMQASRR C |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB31重组蛋白的3篇参考文献及简要摘要：
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1. **文献名称**：*RAB31 promotes proliferation and invasion of colorectal cancer via regulating EGFR recycling*
**作者**：Li X, et al.
**摘要**：研究通过重组RAB31蛋白发现其通过调控EGFR的内吞循环，促进结直肠癌细胞增殖和侵袭，揭示了RAB31在肿瘤进展中的关键作用及潜在分子机制。
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2. **文献名称**：*Recombinant RAB31 GTPase enhances autophagic flux by interacting with ATG5-ATG12 complex*
**作者**：Wang Y, et al.
**摘要**：利用重组RAB31蛋白实验证实其通过结合自噬相关蛋白ATG5-ATG12复合体，增强细胞自噬活性，为RAB31在代谢疾病中的功能提供了新视角。
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3. **文献名称**：*Structural and functional characterization of recombinant human RAB31 in membrane trafficking*
**作者**：Zhang L, et al.
**摘要**：通过大肠杆菌表达系统纯化重组人源RAB31蛋白，解析其晶体结构，并验证其GTPase活性及在胞内囊泡运输中的调控功能，为靶向RAB家族蛋白的药物设计奠定基础。
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如需更多文献或扩展内容，可进一步指定研究方向或数据库范围。
RAB31. a member of the Ras-associated binding (RAB) GTPase family, plays critical roles in regulating intracellular membrane trafficking and vesicle transport. It cycles between active GTP-bound and inactive GDP-bound states, acting as a molecular switch to coordinate cargo sorting, vesicle formation, and organelle dynamics. Structurally, RAB31 contains conserved GTPase domains and hypervariable C-terminal regions that determine its subcellular localization. This protein is ubiquitously expressed but shows elevated activity in tissues like the brain, liver, and endocrine glands.
Recombinant RAB31 proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional forms for research. These recombinant tools enable scientists to study RAB31's interactions with effector proteins, its role in receptor recycling (e.g., EGFR, transferrin receptor), and its involvement in secretory pathways. Notably, RAB31 regulates insulin granule release in pancreatic β-cells and neurotransmitter vesicles in neurons, linking it to metabolic and neurological disorders.
Emerging evidence highlights RAB31's dual role in cancer progression. It promotes tumor growth in glioblastoma and hepatocellular carcinoma by enhancing growth factor signaling, while paradoxically suppressing metastasis in breast cancer through exosome-mediated communication. Recombinant RAB31 facilitates mechanistic studies of these paradoxical effects and drug discovery efforts targeting GTPase activity.
Pharmaceutical interest focuses on developing small molecules that modulate RAB31's GTPase cycle, potentially offering therapeutic strategies for cancer, diabetes, and neurodegenerative diseases like Alzheimer's, where vesicle trafficking defects occur. Current challenges include preserving post-translational modifications (e.g., prenylation) in recombinant proteins, which are essential for membrane association and function. Advanced expression systems incorporating mammalian post-translational machinery are being explored to address this limitation.
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