| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB27A |
| Uniprot No | P51159 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-221aa |
| 氨基酸序列 | SDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGIDFREKRVVYRASGPDGATGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGACGC |
| 预测分子量 | 26.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB27A重组蛋白的3篇代表性参考文献的虚构示例(仅供格式参考，实际文献需通过学术数据库查询)：
1. **文献名称**：*RAB27A regulates exosome secretion through interaction with Slp4 in melanoma cells*
**作者**：Hume AN, et al.
**摘要**：研究利用重组RAB27A蛋白在黑色素瘤细胞中验证其与效应蛋白Slp4的相互作用，揭示RAB27A通过调控溶酶体相关细胞器的分泌促进肿瘤转移的分子机制。
2. **文献名称**：*Recombinant RAB27A expression rescues cytotoxicity defects in Griscelli syndrome patient-derived lymphocytes*
**作者**：Ménasché G, et al.
**摘要**：通过体外表达重组RAB27A蛋白，成功恢复Griscelli综合征患者淋巴细胞的细胞毒性功能，证明RAB27A在免疫突触形成中的关键作用。
3. **文献名称**：*In vitro reconstitution of RAB27A-Munc13-4 complex for vesicle docking studies*
**作者**：Fukuda M, et al.
**摘要**：利用重组RAB27A和Munc13-4蛋白构建体外囊泡锚定模型，阐明二者协同调控血小板致密颗粒分泌的分子机制。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“RAB27A recombinant protein”获取最新研究。
RAB27A is a member of the Rab GTPase family, which regulates intracellular vesicle trafficking, membrane fusion, and exocytosis. It plays a critical role in melanosome transport in melanocytes, lytic granule secretion in cytotoxic T cells, and platelet dense granule release. Mutations in the RAB27A gene are linked to Griscelli syndrome type 2 (GS2) and Hermansky-Pudlak syndrome type 3 (HPS3), rare autosomal recessive disorders characterized by partial albinism, immune dysfunction, and/or bleeding disorders. These conditions underscore RAB27A’s essential function in organelle dynamics and intercellular communication.
Recombinant RAB27A protein is engineered for in vitro studies to dissect its molecular interactions, enzymatic activity, and regulatory mechanisms. Produced via bacterial or mammalian expression systems, the purified protein retains GTPase activity and interacts with effector proteins like Slac2b, synaptotagmin-like proteins (SLPs), and myosin Va. Researchers use it to investigate Rab27a-mediated pathways in secretion, membrane docking, and endosomal sorting. Applications include binding assays, structural studies (e.g., crystallography), and screening for inhibitors or modulators targeting Rab27a-related diseases.
Its role in cancer metastasis and immune regulation has also spurred interest in therapeutic strategies. Recombinant RAB27A enables mechanistic insights into tumor exosome release, immune evasion, and vesicle-based drug delivery systems. By studying post-translational modifications (e.g., prenylation) or GTP/GDP cycling, scientists aim to develop precision therapies for Rab27a-associated pathologies.
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