| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB1B |
| Uniprot No | Q9H0U4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 氨基酸序列 | MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI ELDGKTIKLQ IWDTAGQERF RTITSSYYRG AHGIIVVYDV TDQESYANVK QWLQEIDRYA SENVNKLLVG NKSDLTTKKV VDNTTAKEFA DSLGIPFLET SAKNATNVEQ AFMTMAAEIK KRMGPGAASG GERPNLKIDS TPVKPAGGG |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB1B重组蛋白的3篇参考文献(均为模拟示例，实际文献需自行检索验证)：
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1. **文献名称**：*"Functional characterization of recombinant RAB1B in vesicular transport regulation"*
**作者**：Li, X., et al.
**摘要**：研究通过大肠杆菌系统表达并纯化重组RAB1B蛋白，证实其通过GTP/GDP循环调控内质网-高尔基体间的囊泡运输，并解析了其与效应蛋白相互作用的关键结构域。
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2. **文献名称**：*"RAB1B knockdown disrupts collagen secretion via impaired recombinant protein trafficking"*
**作者**：Zhang, Y., & Wang, H.
**摘要**：利用重组RAB1B突变体进行功能拯救实验，证明RAB1B在胶原蛋白分泌中起关键作用，其活性缺失导致细胞内运输阻滞和分泌缺陷。
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3. **文献名称**：*"Structural insights into RAB1B activation by cryo-EM and biochemical assays"*
**作者**：Smith, J., et al.
**摘要**：通过冷冻电镜解析重组人源RAB1B蛋白与GEF(鸟苷酸交换因子)复合物的三维结构，揭示其激活机制及与疾病相关突变的分子基础。
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注：以上内容为示例，实际文献需通过PubMed、Web of Science或Google Scholar以关键词“RAB1B recombinant”检索获取。
**Background of RAB1B Recombinant Protein**
RAB1B is a member of the Ras-associated binding (RAB) GTPase family, which plays a critical role in regulating intracellular vesicular trafficking. As a small GTPase, RAB1B cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to control membrane dynamics. It is primarily involved in mediating endoplasmic reticulum (ER)-to-Golgi transport, facilitating the docking and fusion of vesicles during early secretory pathways. Dysregulation of RAB1B has been linked to various cellular dysfunctions, including impaired autophagy, disrupted organelle organization, and pathological conditions such as cancer and neurodegenerative diseases.
The recombinant RAB1B protein is engineered for experimental studies to elucidate its structure, function, and interactions. Produced through heterologous expression systems (e.g., *E. coli* or mammalian cells), it is often tagged with affinity markers (e.g., His-tag, GST) for purification and detection. Recombinant RAB1B retains GTPase activity and binding capabilities, enabling in vitro assays to study its interaction with effector proteins, guanine nucleotide exchange factors (GEFs), and GTPase-activating proteins (GAPs).
Research applications include investigating RAB1B’s role in membrane trafficking, organelle dynamics, and disease mechanisms. Its recombinant form is also utilized in structural studies (e.g., crystallography, NMR) to map functional domains and design targeted inhibitors. Additionally, RAB1B serves as a tool to explore crosstalk between vesicular transport and cellular signaling pathways, offering insights into therapeutic strategies for disorders associated with trafficking defects.
Overall, recombinant RAB1B is a vital resource for advancing our understanding of intracellular transport biology and its implications in health and disease.
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