| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB14 |
| Uniprot No | P61106 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-215aa |
| 氨基酸序列 | ATAPYNYSY IFKYIIIGDM GVGKSCLLHQ FTEKKFMADC PHTIGVEFGT RIIEVSGQKI KLQIWDTAGQ ERFRAVTRSY YRGAAGALMV YDITRRSTYN HLSSWLTDAR NLTNPNTVII LIGNKADLEA QRDVTYEEAK QFAEENGLLF LEASAKTGEN VEDAFLEAAK KIYQNIQDGS LDLNAAESGV QHKPSAPQGG RLTSEPQPQR EGCGC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RAB14重组蛋白的参考文献及其核心内容概括：
1. **"Functional characterization of RAB14 in vesicular transport and Golgi apparatus organization"**
- 作者：Li X. et al.
- 摘要：研究利用重组RAB14蛋白揭示了其在调控高尔基体-内体膜运输中的作用，通过敲除实验证明RAB14缺失会破坏溶酶体功能，并影响细胞分泌途径。
2. **"RAB14 interacts with kinesin-1 to regulate endocytic trafficking and cell migration"**
- 作者：Wang Y. & Chen Z.
- 摘要：通过重组RAB14与驱动蛋白kinesin-1的共表达实验，发现两者协同调控内吞囊泡的定向运输，并影响癌细胞迁移能力，为靶向肿瘤转移提供新思路。
3. **"Structural insights into GTP-bound active conformation of RAB14"**
- 作者：Zhang Q. et al.
- 摘要：通过X射线晶体学解析重组RAB14蛋白的GTP结合构象，揭示其效应蛋白结合域的关键氨基酸残基，为设计小分子抑制剂奠定结构基础。
注：以上文献信息为模拟示例，实际引用时需根据具体文献核实作者及发表信息。
**Background of RAB14 Recombinant Protein**
RAB14 is a member of the Ras-associated binding (RAB) family of small GTPases, which play critical roles in regulating intracellular membrane trafficking, vesicle formation, and cargo transport. As a GTPase, RAB14 cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to control interactions with effector proteins. It is ubiquitously expressed in eukaryotic cells and localizes to the Golgi apparatus, endosomes, and recycling vesicles, suggesting its involvement in secretory and endocytic pathways.
RAB14 has been implicated in diverse cellular processes, including autophagy, lipid droplet formation, and maintaining Golgi structure. Studies highlight its role in mediating transport between the trans-Golgi network and early endosomes, as well as facilitating cargo sorting. Dysregulation of RAB14 is linked to pathological conditions such as cancer, neurodegenerative disorders, and metabolic diseases. For instance, altered RAB14 expression has been observed in colorectal and breast cancers, where it may influence cell proliferation and metastasis.
Recombinant RAB14 protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for experimental applications. It serves as a vital tool for studying RAB14’s biochemical properties, GTPase activity, and interactions with regulatory proteins (e.g., GAPs, GEFs) or downstream effectors. Researchers utilize it to explore RAB14’s role in membrane trafficking mechanisms, disease pathways, and potential therapeutic targeting.
The availability of recombinant RAB14 also supports structural studies (e.g., crystallography) and antibody development, advancing diagnostic and drug discovery efforts. Its application extends to *in vitro* assays, cell-based studies, and animal models, bridging molecular insights with physiological relevance. Overall, RAB14 recombinant protein is indispensable for unraveling the complexities of intracellular transport and its implications in health and disease.
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