| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB13 |
| Uniprot No | P51153 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAKAYDHLFKLLLIGDSGVGKTCLIIRFAE DNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRG AMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRK VQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGN GNKPPSTDLKTCDKKNTNKC |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB13重组蛋白的模拟参考文献示例(注：文献信息为虚构，仅用于演示格式)：
1. **文献名称**：*RAB13 regulates tight junction assembly through interaction with JAM-A*
**作者**：Matsuda et al.
**摘要**：本研究解析了重组RAB13蛋白与连接黏附分子JAM-A的相互作用机制，证实RAB13通过GTP酶活性调控上皮细胞紧密连接的形成，并影响细胞极性。
2. **文献名称**：*Recombinant RAB13 expression and its role in cancer cell invasion*
**作者**：Bhuin & Roy
**摘要**：利用重组RAB13蛋白进行功能实验，发现其通过激活侵袭性伪足相关通路促进乳腺癌细胞迁移，为靶向RAB13的癌症治疗提供依据。
3. **文献名称**：*Structural insights into RAB13 GTPase dynamics by cryo-EM*
**作者**：Yadav et al.
**摘要**：通过冷冻电镜解析重组RAB13蛋白的构象变化，揭示其GTP/GDP循环中关键结构域的动态调控机制，为设计小分子抑制剂奠定基础。
4. **文献名称**：*RAB13 knockdown impairs vesicular trafficking in intestinal epithelial cells*
**作者**：Nakamura et al.
**摘要**：利用重组RAB13突变体证明其参与肠上皮细胞囊泡运输，调控Claudin蛋白的膜定位，从而影响肠道屏障完整性。
(提示：实际文献需通过PubMed/Google Scholar检索关键词“RAB13 recombinant protein”“RAB13 function”等获取。)
**Background of RAB13 Recombinant Protein**
RAB13 is a member of the Ras-associated binding (RAB) GTPase family, which plays critical roles in regulating intracellular vesicle trafficking, membrane dynamics, and cell signaling. As a small GTPase, RAB13 cycles between an active GTP-bound state and an inactive GDP-bound state, a switch controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). It is primarily implicated in maintaining cell polarity, tight junction assembly, and cytoskeletal reorganization, particularly in epithelial and endothelial cells.
RAB13 is notably involved in cancer progression and metastasis. Studies highlight its role in promoting epithelial-mesenchymal transition (EMT) by modulating the trafficking of integrins and other adhesion molecules, thereby enhancing cell migration and invasion. Dysregulation of RAB13 has been linked to tumorigenesis in cancers such as breast, colorectal, and glioblastoma.
The recombinant RAB13 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is a vital tool for studying its biochemical and functional properties. Purified recombinant RAB13 retains GTPase activity and interacts with effector proteins, enabling research into its molecular mechanisms. It is widely used in *in vitro* assays, including GTP-binding studies, protein-protein interaction analyses, and screening for therapeutic inhibitors.
Recent advancements in structural biology have utilized recombinant RAB13 to resolve its 3D conformation, providing insights into GTP/GDP binding pockets and regulatory interfaces. Furthermore, its role in neurological disorders, such as neuropathy, is emerging, expanding its relevance beyond oncology.
Overall, RAB13 recombinant protein serves as a key resource for unraveling cellular trafficking pathways and developing targeted therapies for cancer and other diseases linked to RAB GTPase dysfunction.
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