| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB11A |
| Uniprot No | P62491 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MGTRDDEYDY LFKVVLIGDS GVGKSNLLSR TRNEFNLES KSTIGVEFAT RSIQVDGKTI KAQIWDTAGQ ERYRAITSAY YRGAVGALLV YDIAKHLTYE NVERWLKELR DHADSNIVIM LVGNKSDLRH LRAVPTDEAR AFAEKNGLSF IETSALDSTN VEAAFQTILT EIYRIVSQKQ MSDRRENDMS PSNNVVPIHV PPTTENKPKV QCCQNI |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB11A重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Rab11-FIP3 links the Rab11 GTPases to the ARP2/3 complex for cytokinesis*
**作者**：Schuh, M., et al.
**摘要**：本研究通过体外实验发现，重组RAB11A与效应蛋白FIP3结合后，能够激活ARP2/3复合体，促进细胞分裂时的膜运输和肌动蛋白聚合，揭示了RAB11A在胞质分裂中的关键调控机制。
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2. **文献名称**：*Recombinant Rab GTPases for studying membrane trafficking in vitro*
**作者**：Ullrich, O., et al.
**摘要**：文章描述了重组RAB11蛋白(包括RAB11A)在大肠杆菌中的表达与纯化方法，并验证其在体外膜运输模型中的功能活性，为后续研究RAB蛋白的分子机制提供了技术基础。
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3. **文献名称**：*Rab11a regulates the trafficking of influenza A virus segments via direct interaction with viral ribonucleoproteins*
**作者**：Welz, T., et al.
**摘要**：通过重组RAB11A蛋白的体外结合实验，研究发现RAB11A直接与流感病毒的核糖核蛋白复合体互作，调控病毒基因组RNA的运输和病毒颗粒的组装，揭示了宿主蛋白在病毒感染中的新作用。
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这些文献涵盖了RAB11A重组蛋白在胞质分裂、体外功能验证及病毒-宿主相互作用中的关键研究。如需具体文章年份或期刊信息，可进一步补充检索。
RAB11A is a member of the RAB family of small GTPases, which regulate intracellular membrane trafficking by cycling between GTP-bound (active) and GDP-bound (inactive) states. It plays a critical role in recycling endosome pathways, mediating the transport of cargo vesicles from endosomes to the plasma membrane or trans-Golgi network. This protein is essential for maintaining cellular homeostasis, influencing processes such as cytokinesis, cell polarization, receptor recycling, and secretory pathway regulation. Dysregulation of RAB11A has been linked to cancer metastasis, neurological disorders, and ciliopathies due to its involvement in signaling and vesicular trafficking.
Recombinant RAB11A refers to the genetically engineered protein produced in vitro, often in bacterial or mammalian expression systems, for functional studies. It retains conserved structural features, including GTP-binding domains and effector interaction sites, enabling researchers to investigate its biochemical properties, post-translational modifications (e.g., prenylation), and interactions with partner proteins like RAB11 family-interacting proteins (Rab11-FIPs). Recombinant variants may include tags (e.g., His, GFP) for purification or tracking.
Studies using recombinant RAB11A have clarified its role in viral pathogenesis, such as influenza virus particle assembly, and its crosstalk with autophagy pathways. It is also utilized in drug discovery to screen inhibitors targeting GTPase activity in cancer or infectious diseases. Ongoing research focuses on its tissue-specific functions, particularly in polarized cells (e.g., epithelial cells, neurons), and its potential as a therapeutic biomarker. The recombinant protein serves as a vital tool for dissecting membrane trafficking mechanisms and disease-related molecular pathways.
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