| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB10 |
| Uniprot No | P61026 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB10重组蛋白的3篇参考文献示例(文献信息为虚构，仅供示例参考)：
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1. **文献名称**：*Recombinant RAB10 GTPase: Expression, Purification, and Functional Characterization*
**作者**：Chen, X. et al.
**摘要**：该研究报道了在大肠杆菌系统中高效表达并纯化重组人源RAB10蛋白，通过体外GTP水解实验证实其活性，并发现其与效应蛋白EHBP1的相互作用调控细胞内膜运输。
2. **文献名称**：*Structural Insights into RAB10 Activation and Membrane Targeting Mechanisms*
**作者**：Zhang, Y. et al.
**摘要**：利用X射线晶体学解析了RAB10与GDP/GTP类似物的复合物结构，揭示了其构象变化机制，并鉴定了与细胞膜上磷脂分子结合的关键结构域。
3. **文献名称**：*RAB10 Dysregulation in Alzheimer’s Disease: Role of Recombinant Protein in Amyloid Trafficking*
**作者**：Smith, J. et al.
**摘要**：通过哺乳动物细胞表达重组RAB10.发现其过度表达可促进β-淀粉样蛋白前体(APP)的胞内运输异常，提示RAB10在阿尔茨海默症病理中的潜在作用。
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注：以上文献为模拟示例，实际引用时请以真实发表的学术论文为准。建议通过PubMed、Google Scholar等平台以“RAB10 recombinant protein”为关键词检索最新研究。
RAB10 is a member of the RAS superfamily of small GTPases, which play critical roles in regulating intracellular membrane trafficking and vesicle transport. As a key regulator of vesicular dynamics, RAB10 is primarily involved in maintaining cellular polarity, exocytosis, endocytic recycling, and organelle positioning. It cycles between an active GTP-bound state and an inactive GDP-bound state, interacting with effector proteins to coordinate specific trafficking pathways. Studies have linked RAB10 to diverse cellular processes, including insulin-stimulated GLUT4 translocation in adipocytes, axonal outgrowth in neurons, and the regulation of autophagy. Dysregulation of RAB10 has been implicated in several diseases, such as Alzheimer's disease, Parkinson's disease, diabetes, and cancer metastasis.
Recombinant RAB10 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper post-translational modifications. The purified protein retains GTPase activity and structural integrity, enabling functional studies in vitro. Common applications include investigating RAB10's interaction with regulatory proteins (GEFs, GAPs, and effectors), analyzing its role in membrane trafficking mechanisms, and screening therapeutic compounds targeting RAB10-related pathways. Tagged versions (e.g., His, GST, or fluorescent tags) facilitate purification and detection. Recent research highlights its involvement in pathological processes, such as impaired autophagy in neurodegenerative diseases and aberrant Wnt signaling in cancers. The availability of recombinant RAB10 accelerates mechanistic studies and drug discovery efforts focused on membrane trafficking disorders.
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