| 纯度 | >95%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ces2e |
| Uniprot No | Q8BK48 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559aa |
| 氨基酸序列 | MPLYKLLGWLNAVACGVLLLVLHVQGQDSASPIRNTHTGQVRGSLVHVKDTDIAVHTFLGIPFAKPPVGPLRFAPPEAPEPWSGVRDGTSHPNMCLQNDNLMGSEDLKMMNLILPPISMSEDCLYLNIYVPAHAHEGSNLPVMVWIHGGALTVGMASMYDGSMLAATEDVVVVAIQYRLGVLGFFSTGDQHAKGNWGYLDQVAALRWVQQNIVHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVAVLPDLISSSSEMVHRIVANLSGCAAVNSETLMCCLRGKNEAEMLAINKVFKIIPGVVDGEFLPKHPQELMASKDFHPVPSIIGINNDEYGWILPTIMDPAQKIEEITRKTLPAVLKSTALKMMLPPECGDLLMEEYMGDTEDPETLQAQFREMKGDFMFVIPALQVAHFQRSHAPVYFYEFQHRPSFFKDFRPPYVKADHGDEIFLVFGYQFGNIKLPYTEEEEQLSRRIMKYWANFARHGNPNSEGLPYWPVMDHDEQYLQLDIQPSVGRALKARRLQFWTKTLPQKIQELKGSQERHKEL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ces2e重组蛋白的模拟参考文献示例(注：部分内容为假设性概括，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*Expression and Characterization of Recombinant CES2E in a Baculovirus System*
**作者**：Smith A, et al.
**摘要**：本研究利用杆状病毒表达系统成功表达并纯化了Ces2e重组蛋白，分析了其酶动力学参数，证实其对短链脂肪酸酯类底物的高效水解活性。
2. **文献名称**：*Substrate Specificity of Human Carboxylesterase CES2E: Implications for Drug Metabolism*
**作者**：Li X, Wang Y.
**摘要**：通过体外酶活实验，揭示了Ces2e重组蛋白对多种临床药物(如抗病毒前药)的代谢能力，发现其与CES2A亚型存在底物偏好差异。
3. **文献名称**：*Crystal Structure of Recombinant CES2E Unveils Catalytic Mechanism*
**作者**：Tanaka K, et al.
**摘要**：首次解析了Ces2e重组蛋白的晶体结构，阐明了其活性中心关键氨基酸残基在底物识别和水解反应中的分子机制。
4. **文献名称**：*CES2E Knockout and Recombinant Rescue Models in Lipid Homeostasis*
**作者**：Zhang R, et al.
**摘要**：利用基因编辑技术构建Ces2e敲除小鼠模型，并通过重组蛋白回补实验证明Ces2e在肝脏甘油三酯代谢中的调控作用。
**提示**：以上为假设性示例，实际文献需以PubMed/Google Scholar关键词“CES2E recombinant”或“Carboxylesterase 2E”检索确认。
Ces2e (carboxylesterase 2E) is a member of the carboxylesterase enzyme family, which plays critical roles in hydrolyzing ester- and amide-containing compounds, including xenobiotics and endogenous lipids. As a serine hydrolase, it shares structural homology with other CES isoforms, featuring a conserved catalytic triad (Ser-His-Glu) and a characteristic α/β-hydrolase fold. Ces2e is primarily expressed in metabolic tissues such as the liver and intestine, where it participates in Phase I drug metabolism, lipid regulation, and detoxification pathways. Its broad substrate specificity enables interactions with clinical drugs (e.g., chemotherapeutic prodrugs, antivirals), environmental toxins, and lipid-derived signaling molecules.
Recombinant Ces2e protein is engineered using heterologous expression systems like E. coli or mammalian cell lines, often fused with affinity tags (e.g., His-tag) for purification. This technology allows large-scale production of enzymatically active protein for functional studies. Research focuses on its role in drug activation/inactivation kinetics, with implications for personalized medicine. For instance, genetic polymorphisms in CES2E may influence individual responses to prodrugs like irinotecan. Additionally, Ces2e's involvement in lipid metabolism links it to metabolic disorders, driving interest in its regulatory mechanisms. Recombinant variants are also used to screen enzyme inhibitors or environmental pollutants, aiding toxicology assessments. Recent studies explore its potential as a biomarker or therapeutic target in cancers and fatty liver disease. However, functional redundancy among CES isoforms and tissue-specific expression patterns complicate its precise physiological characterization, underscoring the need for isoform-specific tools like recombinant proteins.
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