| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PYGL |
| Uniprot No | P06737 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-847aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSEFELRRQASMAKPLTDQEKRRQISIRG IVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRW IRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGL DIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFN QKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWID TQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVL DRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFG STRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWEL TQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVAL FPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKV FKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQ LTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQV KRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAK MIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQIS TAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDD VAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFY HDRFKVFADYEAYVKCQDKVSQLYMNPKAWNMVLKNIAASGKFSSDRTIK EYAQNIWNVEPSDLKISLSNESNKVNGN |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PYGL重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression and characterization of recombinant human liver glycogen phosphorylase (PYGL) in Escherichia coli"*
**作者**：Chen Y, et al.
**摘要**：该研究报道了人源PYGL基因在大肠杆菌中的重组表达及纯化，证实重组蛋白具有催化糖原分解的活性，为糖尿病治疗靶点研究提供工具。
2. **文献名称**：*"Structural insights into PYGL activation by phosphorylation: implications for hepatocellular carcinoma progression"*
**作者**：Li H, et al.
**摘要**：通过重组PYGL蛋白的磷酸化实验，揭示了其构象变化与肝癌细胞糖代谢异常的关系，为靶向PYGL的抗癌策略提供结构基础。
3. **文献名称**：*"Functional analysis of PYGL mutations in glycogen storage disease type VI using recombinant enzyme models"*
**作者**：Wang X, et al.
**摘要**：利用重组PYGL蛋白模拟VI型糖原累积症相关突变，发现特定突变导致酶活性显著降低，阐明了疾病发生的分子机制。
注：上述文献信息为示例性内容，实际研究中建议通过PubMed或Web of Science以“PYGL recombinant”、“glycogen phosphorylase expression”等关键词检索最新文献。
**Background of PYGL Recombinant Protein**
PYGL (Glycogen Phosphorylase, Liver Form) is a critical enzyme involved in glycogenolysis, the metabolic pathway responsible for breaking down glycogen into glucose-1-phosphate to maintain blood glucose levels. Encoded by the *PYGL* gene in humans, this enzyme is predominantly expressed in the liver and plays a vital role in energy homeostasis, particularly during fasting or intense physical activity. Dysregulation of PYGL activity is linked to glycogen storage diseases (GSDs), such as GSD VI (Hers disease), characterized by hepatomegaly, hypoglycemia, and growth retardation.
Recombinant PYGL protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to enable detailed study of its structure, function, and interactions. The purified protein retains enzymatic activity, allowing researchers to investigate its kinetic properties, allosteric regulation (e.g., by AMP or phosphorylation), and responses to inhibitors. This has facilitated drug discovery efforts targeting PYGL in metabolic disorders, including diabetes and obesity, where modulating glycogen metabolism could offer therapeutic benefits.
Additionally, PYGL recombinant protein serves as a tool for diagnosing GSDs via enzyme activity assays in clinical settings. Its use in structural biology (e.g., X-ray crystallography) has revealed insights into substrate-binding sites and conformational changes during catalysis. Challenges in its production include ensuring proper post-translational modifications (e.g., phosphorylation at Ser15) for full activity, often addressed through optimized expression systems.
Overall, PYGL recombinant protein is a cornerstone for advancing research in glycogen metabolism, metabolic disease mechanisms, and the development of targeted therapies.
×
