| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PVALB |
| Uniprot No | P20472 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-110aa |
| 氨基酸序列 | SMTDLLNAEDIKKAVGAFSATDSFDHKKFFQMVGLKKKSADDVKKVFHMLDKDKSGFIEEDELGFILKGFSPDARDLSAKETKMLMAAGDKDGDGKIGVDEFSTLVAES |
| 预测分子量 | 27.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于PVALB重组蛋白的参考文献示例(实际文献请通过学术数据库查询)：
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1. **标题**: *High-yield expression and purification of recombinant human PVALB in E. coli*
**作者**: Smith J, et al.
**摘要**: 研究利用大肠杆菌表达系统高效生产重组人源PVALB，通过优化诱导条件和亲和层析纯化，获得高纯度蛋白，并验证其钙离子结合活性。
2. **标题**: *Structural and functional characterization of recombinant zebrafish parvalbumin*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析重组斑马鱼PVALB的三维结构，揭示其钙结合域的动态变化，并比较其与哺乳动物同源物的功能差异。
3. **标题**: *Recombinant PVALB suppresses epileptiform activity in neuronal networks*
**作者**: Zhang Y, et al.
**摘要**: 在体外神经元模型中，重组PVALB通过调节细胞内钙稳态，显著抑制癫痫样放电，提示其作为神经保护剂的潜力。
4. **标题**: *Development of PVALB-specific antibodies using recombinant protein as immunogen*
**作者**: Brown K, et al.
**摘要**: 以重组大鼠PVALB为抗原制备单克隆抗体，成功应用于脑组织免疫组化，为神经疾病标记物研究提供工具。
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**说明**：以上内容为模拟生成，实际研究需参考PubMed、Web of Science等平台的文献。建议结合关键词“recombinant parvalbumin”、“PVALB expression”等进行检索。
**Background of PVALB Recombinant Protein**
Parvalbumin (PVALB) is a calcium-binding protein belonging to the EF-hand protein family, widely expressed in vertebrates. It plays critical roles in regulating intracellular calcium homeostasis, modulating neuronal excitability, and influencing muscle contraction-relaxation cycles. Structurally, PVALB contains three EF-hand domains, two of which actively bind calcium ions, enabling it to buffer calcium and participate in signal transduction pathways.
Recombinant PVALB refers to the protein produced through genetic engineering techniques, typically by expressing the PVALB gene in heterologous systems such as *E. coli*, yeast, or mammalian cell lines. This approach ensures high purity, scalability, and consistency, overcoming limitations associated with isolating the protein from native tissues. Recombinant PVALB retains the functional properties of its natural counterpart, including calcium-binding affinity and structural stability, making it a valuable tool for biochemical and biomedical research.
In neuroscience, PVALB is a marker for fast-spiking GABAergic interneurons, and its dysregulation is linked to neurological disorders such as epilepsy, schizophrenia, and autism spectrum disorders. Recombinant PVALB facilitates studies on calcium signaling mechanisms, synaptic plasticity, and disease models. In muscular systems, it aids in investigating conditions like myopathies or cardiac arrhythmias. Additionally, recombinant PVALB is utilized in antibody production, diagnostic assays, and drug development due to its immunogenicity and well-characterized structure.
Overall, recombinant PVALB serves as a crucial reagent for dissecting calcium-mediated processes and advancing therapeutic strategies for related pathologies. Its engineered production bridges gaps between basic research and clinical applications, highlighting its significance in modern molecular biology.
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