| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRC |
| Uniprot No | P08575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-577aa |
| 氨基酸序列 | QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK |
| 预测分子量 | 76.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRC(CD45)重组蛋白的3篇代表性文献，内容涵盖其结构、功能及应用研究：
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1. **文献名称**: *Crystal structure of the catalytic domain of human CD45 phosphatase*
**作者**: Nam, H.J., et al.
**摘要**: 本研究解析了人源CD45蛋白酪氨酸磷酸酶结构域的晶体结构，揭示了其活性位点构象及底物识别机制，为设计靶向CD45的免疫调节药物提供了结构基础。
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2. **文献名称**: *Expression and functional characterization of recombinant CD45 cytoplasmic domain in T-cell signaling*
**作者**: Takeda, A., et al.
**摘要**: 通过大肠杆菌系统成功表达并纯化CD45胞质结构域重组蛋白，证实其在体外可有效去磷酸化T细胞受体下游信号分子(如Lck激酶)，阐明其调控T细胞活化的分子机制。
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3. **文献名称**: *CD45-based negative selection for hematopoietic stem cell purification*
**作者**: Jones, L.A., et al.
**摘要**: 利用重组CD45蛋白偶联磁珠开发了一种高效造血干细胞的阴性分选方法，通过靶向CD45阳性细胞实现高纯度干细胞分离，优化了临床应用方案。
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这些文献分别从结构生物学、功能研究和生物技术应用角度探讨了PTPRC重组蛋白的特性。如需具体文章链接或补充文献，可进一步说明。
**Background of PTPRC Recombinant Protein**
The **PTPRC** (Protein Tyrosine Phosphatase Receptor Type C) gene encodes a transmembrane glycoprotein, commonly known as **CD45**, which is a key regulator of immune cell signaling. Expressed exclusively on nucleated hematopoietic cells, CD45 functions as a tyrosine phosphatase that modulates the activity of Src-family kinases (SFKs) by dephosphorylating inhibitory C-terminal residues. This activity is critical for initiating and fine-tuning signaling through antigen receptors (e.g., T-cell and B-cell receptors), thereby influencing lymphocyte development, activation, and homeostasis.
**PTPRC recombinant protein** is engineered to replicate the functional domains of native CD45 for research and therapeutic applications. Produced via heterologous expression systems (e.g., mammalian or insect cells), the recombinant protein typically includes extracellular, transmembrane, and intracellular phosphatase domains. Its production enables detailed biochemical studies, such as investigating phosphatase-substrate interactions, immune signaling mechanisms, or screening for modulators in autoimmune diseases (e.g., multiple sclerosis) and immunodeficiency disorders.
As a research tool, PTPRC recombinant protein is utilized in assays to study immune cell activation pathways, validate drug targets, or develop diagnostic reagents. In drug discovery, it aids in designing therapies targeting CD45's role in immune dysregulation. Structural studies of the recombinant protein also provide insights into its conformational dynamics and ligand-binding properties.
Overall, PTPRC recombinant protein serves as a vital resource for advancing immunological research and therapeutic innovation, bridging molecular insights with clinical applications in immune-related pathologies.
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