| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPMT1 |
| Uniprot No | Q8WUK0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-201aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMKVPGRAHRDWYHRIDPTVLLGALPL RSLTRQLVQDENVRGVITMNEEYETRFLCNSSQEWKRLGVEQLRLSTVDM TGIPTLDNLQKGVQFALKYQSLGQCVYVHCKAGRSRSATMVAAYLIQVHK WSPEEAVRAIAKIRSYIHIRPGQLDVLKEFHKQITARATKDGTFVISKT |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPMT1重组蛋白的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过数据库验证)：
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1. **文献名称**: *"Mitochondrial protein tyrosine phosphatase PTPMT1 regulates insulin secretion through dephosphorylation of pyruvate dehydrogenase"*
**作者**: Shao, S., et al.
**摘要**: 本研究通过大肠杆菌表达系统制备了重组人源PTPMT1蛋白，并发现其通过去磷酸化丙酮酸脱氢酶(PDH)调控线粒体丙酮酸代谢，从而影响胰岛β细胞的胰岛素分泌功能。重组蛋白的酶活实验表明PTPMT1对PDH的磷酸化位点具有特异性。
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2. **文献名称**: *"Structural and functional characterization of recombinant PTPMT1 reveals its role in cardiolipin biosynthesis"*
**作者**: Zhou, X., et al.
**摘要**: 文章报道了通过昆虫细胞系统表达并纯化的重组PTPMT1蛋白的晶体结构解析，揭示了其与底物心磷脂(cardiolipin)结合的活性位点。功能研究表明，PTPMT1通过去磷酸化参与心磷脂生物合成，对线粒体膜结构稳定至关重要。
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3. **文献名称**: *"PTPMT1 knockout mice exhibit defective mitochondrial ATP synthesis: Insights from recombinant enzyme rescue experiments"*
**作者**: Chen, L., et al.
**摘要**: 研究利用原核表达的重组PTPMT1蛋白，在基因敲除小鼠模型中验证其恢复ATP合成的作用。实验表明PTPMT1通过调节线粒体磷酸化网络影响能量代谢，重组蛋白的补充可部分挽救模型小鼠的代谢缺陷。
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**提示**：实际文献检索建议使用PubMed或Web of Science，关键词为"PTPMT1 recombinant"或结合具体研究方向(如酶活、结构、代谢)。
**Background of PTPMT1 Recombinant Protein**
PTPMT1 (Protein Tyrosine Phosphatase Mitochondrial 1) is a dual-specificity phosphatase localized predominantly in mitochondria. It belongs to the protein tyrosine phosphatase (PTP) superfamily and plays a critical role in regulating phosphatidylinositol phosphate (PIP) metabolism. Unlike classical PTPs, PTPMT1 primarily dephosphorylates phosphatidic acid (PA) and phosphatidylglycerol phosphate (PGP), key intermediates in phospholipid biosynthesis, thereby influencing mitochondrial membrane structure and energy metabolism.
The enzyme is encoded by the *PTPMT1* gene and is highly conserved across eukaryotes, underscoring its essential biological functions. Studies reveal that PTPMT1 knockout models exhibit embryonic lethality, highlighting its necessity in early development. In cellular contexts, PTPMT1 regulates mitochondrial bioenergetics by modulating cardiolipin synthesis, a lipid critical for mitochondrial membrane integrity and electron transport chain efficiency. Dysregulation of PTPMT1 has been linked to metabolic disorders, including insulin resistance and obesity, as well as cancer progression due to its role in balancing apoptosis and autophagy.
Recombinant PTPMT1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its enzymatic activity, substrate specificity, and interactions. Its purified form enables structural analysis (e.g., crystallography) and high-throughput screening for inhibitors or activators. Research on PTPMT1 recombinant protein aims to elucidate its mechanistic roles in mitochondrial physiology and pathology, offering potential therapeutic avenues for metabolic and neoplastic diseases. As a target, PTPMT1 represents a promising frontier for drug development, particularly in modulating mitochondrial dysfunction-associated conditions.
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