| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTP4A1 |
| Uniprot No | Q93096 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-170aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMARMNRPAPVEVTYKNMRFLITHNPTNATL NKFIEELKKYGVTTIVRVCEATYDTTLVEKEGIHVLDWPFDDGAPPSNQI VDDWLSLVKIKFREEPGCCIAVHCVAGLGRAPVLVALALIEGGMKYEDAV QFIRQKRRGAFNSKQLLYLEKYRPKMRLRFKDSNGHRNNC |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTP4A1重组蛋白的相关文献摘要概括(部分信息为模拟整合，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*PTP4A1 promotes tumor cell proliferation and metastasis through ERK signaling in colorectal cancer*
**作者**：Zhang Y, et al.
**摘要**：该研究通过大肠癌细胞模型，利用重组PTP4A1蛋白验证其磷酸酶活性，发现其通过激活ERK信号通路促进肿瘤细胞增殖和转移，为靶向治疗提供依据。
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2. **文献名称**：*Structural and functional analysis of recombinant human PTP4A1 phosphatase*
**作者**：Wang L, et al.
**摘要**：研究团队成功表达并纯化重组人源PTP4A1蛋白，通过X射线晶体学解析其三维结构，结合酶动力学实验揭示其底物特异性，为开发选择性抑制剂奠定基础。
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3. **文献名称**：*PTP4A1 regulates angiogenesis via interaction with VEGF receptor-2*
**作者**：Chen X, et al.
**摘要**：利用重组PTP4A1蛋白进行体外结合实验，证明其直接与VEGFR2相互作用，通过去磷酸化调控血管生成信号通路，提示其在抗血管生成治疗中的潜在价值。
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**提示**：以上文献信息为示例性质，实际研究中建议通过PubMed、Web of Science等平台以“PTP4A1 recombinant protein”“PTP4A1 phosphatase”等关键词检索最新文献。
Phosphatase of regenerating liver 1 (PTP4A1/PRL-1) is a member of the protein tyrosine phosphatase (PTP) superfamily, distinguished by its unique role in cellular signaling and association with cancer progression. Unlike classical PTPs that dephosphorylate tyrosine residues, PRL-1 exhibits dual-specificity phosphatase activity, targeting both phosphotyrosine and phosphoserine/threonine substrates. It is encoded by the *PTP4A1* gene and is part of the PRL family, which includes PRL-2 and PRL-3. These phosphatases share a conserved C-terminal catalytic PTP domain and an N-terminal prenylation motif critical for membrane localization.
PRL-1 is implicated in regulating cell proliferation, migration, and metastasis, primarily through interactions with Rho GTPases, MAPK, and PI3K/Akt pathways. Its overexpression in cancers (e.g., colorectal, liver, breast) correlates with poor prognosis, likely due to enhanced extracellular matrix remodeling and epithelial-mesenchymal transition. PRL-1 also influences endosomal trafficking and receptor tyrosine kinase signaling, further linking it to oncogenic processes.
Recombinant PTP4A1 protein is produced via heterologous expression systems (e.g., *E. coli*, mammalian cells) for functional studies. It retains enzymatic activity and structural integrity, enabling in vitro assays to probe substrate specificity, screen inhibitors, or study protein-protein interactions. Tagged versions (e.g., His-, GST-tagged) facilitate purification and detection. Researchers use recombinant PRL-1 to explore its role in disease models, validate its catalytic mechanisms, and develop targeted therapies. Despite its established oncogenic role, emerging evidence suggests PRL-1 may also function in neuronal development and metabolic regulation, highlighting its diverse biological relevance.
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