| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN4 |
| Uniprot No | P29074 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 655-913aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMVLTQFDQLYRKKPGMTMSCAKLPQNISKN RYRDISPYDATRVILKGNEDYINANYINMEIPSSSIINQYIACQGPLPHT CTDFWQMTWEQGSSMVVMLTTQVERGRVKCHQYWPEPTGSSSYGCYQVTC HSEEGNTAYIFRKMTLFNQEKNESRPLTQIQYIAWPDHGVPDDSSDFLDF VCHVRNKRAGKEEPVVVHCSAGIGRTGVLITMETAMCLIECNQPVYPLDI VRTMRDQRAMMIQTPSQYRFVCEAILKVYE |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPN4重组蛋白的参考文献示例(注：内容为虚构，仅作格式参考)：
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1. **标题**：*Expression and Purification of Recombinant PTPN4 in Escherichia coli for Functional Studies*
**作者**：Smith A, et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备了重组PTPN4蛋白，通过优化诱导条件和层析纯化步骤，获得了高纯度且具有磷酸酶活性的蛋白，为后续酶学分析提供了基础。
2. **标题**：*Crystal Structure of the Catalytic Domain of PTPN4 Reveals Substrate Specificity Mechanisms*
**作者**：Jones B, et al.
**摘要**：通过X射线晶体学解析了PTPN4催化结构域的三维结构，揭示了其底物结合口袋的关键氨基酸残基，阐明了其对特定酪氨酸磷酸化位点的选择性去磷酸化机制。
3. **标题**：*PTPN4 Regulates Neuronal Synaptic Plasticity via Interaction with Glutamate Receptors*
**作者**：Lee C, et al.
**摘要**：利用重组PTPN4蛋白进行体外实验，发现其通过去磷酸化AMPA受体亚基GluA1调控突触传递，提示PTPN4在神经可塑性和学习记忆中的潜在作用。
4. **标题**：*Role of PTPN4 in T-Cell Receptor Signaling and Autoimmunity*
**作者**：Chen D, et al.
**摘要**：通过重组PTPN4蛋白的体外磷酸酶活性分析，证明其负向调控T细胞受体信号通路，抑制过度免疫反应，为自身免疫疾病治疗提供了新靶点。
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如需查找真实文献，建议在 **PubMed** 或 **Google Scholar** 中搜索关键词：
`PTPN4 recombinant protein expression`、`PTPN4 structure-function`、`PTPN4 phosphatase activity`。
**Background of PTPN4 Recombinant Protein**
PTPN4 (Protein Tyrosine Phosphatase Non-Receptor Type 4), also known as PTP-MEG1. is a member of the protein tyrosine phosphatase (PTP) family, which plays a critical role in regulating cellular signaling by dephosphorylating tyrosine residues on target proteins. This enzyme is characterized by a conserved catalytic phosphatase domain and a unique PDZ (Postsynaptic Density-95/Discs Large/Zonula Occludens-1) domain, which facilitates protein-protein interactions and substrate recognition. PTPN4 is widely expressed in various tissues, with notable roles in immune response modulation, neuronal development, and apoptosis regulation.
Recombinant PTPN4 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional enzyme for research. Its production often involves affinity tag-based purification (e.g., GST or His-tag) to ensure high yield and specificity. Studies highlight PTPN4’s involvement in T-cell receptor signaling, where it acts as a negative regulator to prevent excessive immune activation. Dysregulation of PTPN4 has been implicated in autoimmune diseases, neurological disorders (e.g., Alzheimer’s disease), and cancer, though its exact mechanisms remain under investigation.
In cancer, PTPN4 exhibits dual roles—acting as a tumor suppressor in some contexts (e.g., by inhibiting oncogenic kinases) or promoting tumor progression in others. Its PDZ domain may interact with viral proteins or cellular effectors, linking it to pathogen-host interactions. Recombinant PTPN4 is pivotal for *in vitro* studies to dissect substrate specificity, enzymatic activity, and signaling pathways. Additionally, it serves as a tool for drug discovery, aiming to develop modulators targeting PTPs, a challenging class due to their conserved active sites. Ongoing research continues to unravel PTPN4’s therapeutic potential and pathophysiological relevance.
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