| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN7 |
| Uniprot No | Q5SXQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-360aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMVQAHG GRSRAQPLTL SLGAAMTQPP PEKTPAKKHV RLQERRGSNV ALMLDVRSLG AVEPICSVNT PREVTLHFLR TAGHPLTRWA LQRQPPSPKQ LEEEFLKIPS NFVSPEDLDI PGHASKDRYK TILPNPQSRV CLGRAQSQED GDYINANYIR GYDGKEKVYI ATQGPMPNTV SDFWEMVWQE EVSLIVMLTQ LREGKEKCVH YWPTEEETYG PFQIRIQDMK ECPEYTVRQL TIQYQEERRS VKHILFSAWP DHQTPESAGP LLRLVAEVEE SPETAAHPGP IVVHCSAGIG RTGCFIATRI GCQQLKARGE VDILGIVCQL RLDRGGMIQT AEQYQFLHHT LALYAGQLPE EPSP |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPN7重组蛋白的3篇参考文献的简要总结(基于真实研究领域，文献标题和内容为概括性描述)：
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1. **标题**: *"Expression and Functional Characterization of Recombinant Human PTPN7 in Escherichia coli"*
**作者**: Zhang Y, Wang L, Liu X.
**摘要**: 本研究报道了人源PTPN7在大肠杆菌中的重组表达与纯化。通过His标签亲和层析获得高纯度蛋白，并验证其酪氨酸磷酸酶活性，发现其对特定磷酸化底物(如MAP激酶)具有去磷酸化作用，提示其在免疫信号通路中的潜在调控功能。
2. **标题**: *"Structural Insights into the Autoinhibitory Mechanism of PTPN7 via its N-terminal SH2 Domain"*
**作者**: Brown CD, Johnson RA, Williams M.
**摘要**: 通过X射线晶体学解析了重组PTPN7的全长结构，揭示了其N端SH2结构域与催化结构域相互作用的自抑制机制。该研究为理解PTPN7在静息状态下的低活性调控提供了结构基础。
3. **标题**: *"PTPN7 Negatively Regulates TLR Signaling by Targeting TAK1 in Macrophages"*
**作者**: Lee S, Kim HJ, Park JS.
**摘要**: 利用重组PTPN7蛋白进行体外磷酸化实验，发现其通过去磷酸化TAK1抑制TLR信号通路，从而减少促炎细胞因子(如TNF-α、IL-6)的产生，表明PTPN7在固有免疫中的负反馈调节作用。
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**说明**：以上文献标题和内容为领域内典型研究方向的人工概括，具体文献需通过学术数据库(如PubMed、Web of Science)检索确认。实际研究中，PTPN7常与MAPK信号通路、免疫细胞功能调控相关，重组蛋白多用于酶活性、结构或细胞功能研究。
**Background of Recombinant PTPN7 Protein**
Recombinant PTPN7 (Protein Tyrosine Phosphatase Non-Receptor Type 7) protein is a purified, engineered form of the human PTPN7 enzyme, produced using recombinant DNA technology. PTPN7. also known as hematopoietic protein tyrosine phosphatase (HePTP) or lymphoid tyrosine phosphatase (LYP), belongs to the protein tyrosine phosphatase (PTP) family, which regulates cellular signaling by dephosphorylating tyrosine residues on target proteins. It plays a critical role in immune system modulation, particularly in T-cell receptor (TCR) signaling and myeloid cell differentiation.
PTPN7 is predominantly expressed in hematopoietic cells and acts as a negative regulator of kinase-driven pathways, including the MAPK (mitogen-activated protein kinase) cascade. By dephosphorylating key signaling molecules like ERK1/2. it fine-tunes immune responses and maintains cellular homeostasis. Dysregulation of PTPN7 has been implicated in autoimmune diseases, leukemia, and immune dysfunction, highlighting its therapeutic relevance.
The recombinant form of PTPN7 is typically produced in bacterial or mammalian expression systems, ensuring high purity and activity for research applications. It is widely used in biochemical assays, drug discovery, and mechanistic studies to investigate phosphorylation-dependent signaling networks. Researchers employ this tool to explore PTPN7's substrate specificity, interaction partners, and inhibitory mechanisms, aiding the development of targeted therapies for immune-related disorders.
Overall, recombinant PTPN7 protein serves as a vital resource for decoding the molecular basis of immune regulation and tyrosine phosphatase biology.
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