| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | QTRT1 |
| Uniprot No | Q9BXR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSEFMAGAATQASLESAPRIMRLVAECSR SRARAGELWLPHGTVATPVFMPVGTQATMKGITTEQLDALGCRICLGNTY HLGLRPGPELIQKANGLHGFMNWPHNLLTDSGGFQMVSLVSLSEVTEEGV RFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDDVVSSTVTGPRVEEAM YRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMTKRDVPGF AIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGC DMFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKH SRAFLHALLHSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPDFVRD FMGAMYGDPTLCPTWATDALASVGITLG |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于QTRT1重组蛋白的3篇代表性文献示例及简要概括：
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1. **文献名称**：*Structural insights into the catalytic mechanism of human QTRT1 in tRNA modification*
**作者**：Smith J, et al.
**摘要**：本研究通过X射线晶体学解析了重组人源QTRT1蛋白的三维结构，揭示了其结合底物queuine和tRNA的关键结构域，阐明了其催化tRNA修饰的分子机制，并验证了活性位点突变对酶活性的影响。
2. **文献名称**：*Heterologous expression and functional characterization of recombinant QTRT1 in E. coli*
**作者**：Chen L, et al.
**摘要**：作者构建了大肠杆菌表达系统，成功纯化出高活性的重组QTRT1蛋白，并通过体外酶活实验证明其能特异性催化tRNA的queuine插入，为后续生化研究提供了可靠工具。
3. **文献名称**：*QTRT1 deficiency disrupts tRNA modification and links to neurodevelopmental disorders*
**作者**：Garcia-Rodriguez N, et al.
**摘要**：该研究利用基因编辑技术构建QTRT1缺失细胞模型，发现重组QTRT1蛋白回补可恢复tRNA修饰水平，并证实其功能异常与神经发育障碍中异常的蛋白质翻译调控相关。
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注：以上文献为假设性示例，实际研究中建议通过PubMed或Google Scholar检索具体文献(关键词：QTRT1 recombinant, queuine tRNA-ribosyltransferase)。
**Background of QTRT1 Recombinant Protein**
QTRT1 (queuine tRNA-ribosyltransferase catalytic subunit 1) is an evolutionarily conserved enzyme involved in the post-transcriptional modification of transfer RNA (tRNA). It catalyzes the insertion of queuine, a hypermodified base, into the wobble position of specific tRNAs (tRNAᵁᴰᵁ, tRNAᴬˢᴺ, tRNAᴴⁱˢ, and tRNAᵀʸʳ), replacing guanine to form queuosine (Q). This modification enhances translational fidelity, efficiency, and stress adaptation in eukaryotes. QTRT1 operates in complex with its paralog QTRT2. forming a heterodimer critical for enzymatic activity.
The recombinant QTRT1 protein is typically produced in *E. coli* or mammalian expression systems, enabling studies on its structure, function, and interaction partners. Its recombinant form retains the ability to bind queuine and tRNAs, facilitating *in vitro* assays to dissect the queuosinylation mechanism. Structural analyses reveal conserved domains, including a catalytic TGS (ThrRS, GTPase, and SpoT) fold, which coordinates substrate binding and ribosyltransferase activity.
Dysregulation of QTRT1 is linked to developmental defects, neurological disorders, and cancer progression, underscoring its role in cellular homeostasis. Recombinant QTRT1 serves as a tool to explore these associations, model mutations, and screen potential therapeutics. Additionally, it aids in studying queuine’s role as a micronutrient and its impact on gene expression.
In summary, QTRT1 recombinant protein bridges molecular biology and disease research, offering insights into tRNA modification mechanisms and their broader implications in health and disease.
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