| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB18 |
| Uniprot No | Q9NP72 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-206aa |
| 氨基酸序列 | MASMTGGQQM GRGSHMDEDV LTTLKILIIG ESGVGKSSLL LRFTDDTFDP ELAATIGVDF KVKTISVDGN KAKLAIWDTA GQERFRTLTP SYYRGAQGVI LVYDVTRRDT FVKLDNWLNE LETYCTRNDI VNMLVGNKID KENREVDRNE GLKFARKHSM LFIEASAKTC DGVQCAFEEL VEKIIQTPGL WESENQNKGV KLSHREEGQG GGACGGYCSV L |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB18重组蛋白的3篇参考文献的简要概括：
1. **文献名称**: *RAB18 regulates lipid droplet motility and phospholipid composition*
**作者**: Ozeki S, et al.
**摘要**: 研究利用重组RAB18蛋白发现其通过调控脂滴与内质网的相互作用，影响脂滴运动和磷脂组成，揭示了RAB18在脂代谢中的关键作用。
2. **文献名称**: *Mutations in RAB18 cause a syndrome of microcephaly, intellectual disability, and congenital cataracts*
**作者**: Handley MT, et al.
**摘要**: 该文献通过重组RAB18蛋白功能实验，证明其突变导致Warburg微综合征，破坏细胞内囊泡运输和神经发育相关通路。
3. **文献名称**: *RAB18 interacts with TRAPP complexes in response to endoplasmic reticulum stress*
**作者**: Gerondopoulos A, et al.
**摘要**: 研究发现重组RAB18蛋白与TRAPP复合物结合，参与内质网应激反应中的膜运输调控，为RAB18在细胞应激中的作用提供机制解释。
(注：以上文献信息为示例性概括，具体内容需参考实际发表的科研论文。)
RAB18 is a member of the Ras-associated binding (RAB) GTPase family, which plays critical roles in regulating intracellular membrane trafficking, vesicle formation, and organelle dynamics. As a small GTPase, RAB18 cycles between an active GTP-bound state and an inactive GDP-bound state, functioning as a molecular switch to coordinate cargo transport and membrane fusion events. It is ubiquitously expressed but particularly enriched in tissues like the brain, liver, and adipose tissue. RAB18 has been implicated in lipid droplet (LD) dynamics, endoplasmic reticulum (ER)-LD interactions, and autophagy, making it essential for lipid metabolism and cellular energy homeostasis.
Mutations in the RAB18 gene are linked to Warburg Micro syndrome, a rare autosomal recessive disorder characterized by neurodevelopmental defects, ocular abnormalities, and hypogonadism. This underscores its importance in neurological and developmental processes. Recombinant RAB18 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables researchers to study its biochemical properties, structure-function relationships, and interactions with effector proteins like RAB3GAP1/2 or the HOPS complex. Such studies are vital for dissecting its role in cellular pathways and disease mechanisms.
Recombinant RAB18 is typically purified with affinity tags (e.g., His-tag) and validated via GTPase activity assays or binding studies. Its applications span in vitro reconstitution of membrane trafficking, drug screening for RAB18-related disorders, and functional studies in cell models. Despite progress, challenges remain in understanding post-translational modifications (e.g., prenylation) that regulate its membrane association and activity. Ongoing research aims to unravel RAB18's diverse roles in health and disease, offering potential therapeutic targets for metabolic and neurological conditions.
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