| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB1A |
| Uniprot No | P62820 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-205aa |
| 氨基酸序列 | SSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC |
| 预测分子量 | 49.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB1A重组蛋白的3篇代表性文献摘要，涵盖结构、功能和疾病机制研究：
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1. **文献名称**：*Crystal structure of the human RAB1A protein reveals critical interactions with GTP*
**作者**：S. Yoshimura, A. Nakamura, K. Fukuda
**摘要**：该研究通过重组表达人源RAB1A蛋白，利用X射线晶体学解析了其与GDP/GTP结合的构象差异，揭示了Switch I/II结构域在GTP水解中的关键作用，为理解RAB1A的分子机制提供结构基础。
2. **文献名称**：*RAB1A promotes colorectal cancer metastasis by regulating TGF-β1 secretion via COPII vesicles*
**作者**：Y. Xu, H. Zhang, L. Chen
**摘要**：研究通过重组RAB1A蛋白进行体外囊泡运输实验，证明其通过调控COPII复合体介导的TGF-β1分泌，促进结直肠癌细胞侵袭转移，为靶向RAB1A的癌症治疗提供依据。
3. **文献名称**：*Recombinant RAB1A GTPase activity modulation by Legionella pneumophila effector proteins*
**作者**：M. Ingmundson, C. R. Roy
**摘要**：该研究在大肠杆菌中表达并纯化RAB1A重组蛋白，发现嗜肺军团菌效应蛋白DrrA通过调控其GTP/GDP循环劫持宿主细胞膜运输系统，揭示病原体逃逸宿主防御的机制。
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这些文献涉及RAB1A重组蛋白在结构解析、肿瘤信号转导及病原体互作中的关键作用，均通过重组表达技术探究其功能。如需具体文章，建议通过PubMed以“RAB1A recombinant protein”为关键词检索。
RAB1A is a member of the Ras-associated binding (RAB) GTPase family, which plays a critical role in regulating intracellular vesicle trafficking. As a small GTPase, it cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to control membrane transport pathways. Specifically, RAB1A is essential for endoplasmic reticulum (Golgi-mediated vesicle budding and fusion, facilitating protein secretion and maintaining organelle integrity. It interacts with effector proteins like p115 and GM130 to mediate tethering and docking of transport vesicles, ensuring cargo sorting and directional movement.
Recombinant RAB1A protein is engineered for in vitro studies to dissect its biochemical and structural functions. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant form retains native GTPase activity and binding capabilities, enabling research into its interaction networks, regulatory mechanisms, and post-translational modifications (e.g., prenylation). Its purification often involves affinity tags (e.g., His-tag) to ensure high yield and purity.
Dysregulation of RAB1A is linked to diseases, including cancer, neurodegenerative disorders (e.g., Parkinson’s), and microbial infections. Overexpression of RAB1A has been observed in certain tumors, correlating with enhanced cell proliferation and metastasis. In neurodegeneration, impaired RAB1A function disrupts autophagy-lysosomal pathways, contributing to protein aggregation. Additionally, pathogens like hepatitis C virus hijack RAB1A-dependent trafficking for replication.
Research using recombinant RAB1A aids in developing targeted therapies, such as GTPase inhibitors or modulators of membrane trafficking. Its structural analysis (e.g., X-ray crystallography) provides insights into GTPase dynamics, aiding drug design. Overall, recombinant RAB1A serves as a vital tool for unraveling cellular logistics and disease mechanisms.
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