| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB23 |
| Uniprot No | Q9ULC3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MLEEDMEVAI KMVVVGNGAV GKSSMIQRYC KGIFTKDYKK TIGVDFLERQ IQVNDEDVRL MLWDTAGQEE FDAITKAYYR GAQACVLVFS TTDRESFEAV SSWREKVVAE VGDIPTVLVQ NKIDLLDDSC IKNEEAEALA KRLKLRFYRT SVKEDLNVNE VFKYLAEKYL QKLKQQIAED PELTHSSSNK IGVFNTSGGS HSGQNSGTLN GGDVINLRPN KQRTKKNRNP FSSC |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Rab23 regulates Hedgehog signaling by controlling the distribution of Smoothened"**
*作者：Eggenschwiler, J.T., et al.*
摘要：研究揭示了RAB23在小鼠Hedgehog信号通路中的调控作用，通过重组蛋白实验证实其通过影响Smoothened蛋白的细胞内定位来抑制信号传导，与胚胎发育异常相关。
2. **"Rab23’s role in hepatocellular carcinoma progression through MAPK pathway modulation"**
*作者：Chi, S., et al.*
摘要：利用重组RAB23蛋白分析其在肝癌细胞中的功能，发现其过表达通过激活MAPK信号通路促进肿瘤侵袭，提示其作为潜在治疗靶点。
3. **"Recombinant Rab23 GTPase: Purification and characterization of its interaction with transport vesicles"**
*作者：Lodhi, I.J., et al.*
摘要：描述了RAB23重组蛋白的体外纯化方法及生化特性，证明其通过GTP结合状态调控囊泡运输，为膜运输机制研究提供工具。
4. **"Rab23 mutations in Carpenter syndrome disrupt neural tube patterning"**
*作者：Chen, W., et al.*
摘要：通过构建突变型RAB23重组蛋白，发现其功能缺失导致神经管发育缺陷，关联于Carpenter综合征，强调了其在细胞极性建立中的作用。
RAB23 is a member of the Ras-associated binding (RAB) family of small GTPases, which play critical roles in regulating intracellular vesicle trafficking, membrane dynamics, and organelle organization. Discovered in 1994. RAB23 is evolutionarily conserved and ubiquitously expressed in mammalian tissues. As a GTPase, it cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to coordinate cargo transport and membrane fusion events. Structurally, RAB23 contains conserved GTP-binding domains and a hypervariable C-terminal region that directs its subcellular localization.
Functionally, RAB23 is best known for its role in regulating sonic hedgehog (SHH) signaling, a pathway essential for embryonic development, particularly in neural tube patterning and limb formation. Studies in mice revealed that RAB23 acts as a negative regulator of SHH signaling, with loss-of-function mutations leading to severe developmental defects. Additionally, RAB23 is implicated in autophagy, endosomal sorting, and ciliogenesis, processes linked to cellular homeostasis and disease.
Dysregulation of RAB23 is associated with human pathologies. Recessive mutations in RAB23 cause Carpenter syndrome, a rare genetic disorder characterized by craniofacial abnormalities, polydactyly, and obesity. Conversely, overexpression of RAB23 has been observed in certain cancers, including gliomas and breast cancer, where it may influence tumor progression through interactions with oncogenic pathways.
Recombinant RAB23 protein, typically produced in bacterial or mammalian expression systems, retains GTPase activity and post-translational modifications (e.g., prenylation) when purified. It serves as a vital tool for biochemical studies, including GTP-binding assays, interaction partner identification (e.g., with DISP1 or other trafficking mediators), and screening for small-molecule modulators. Its applications extend to structural studies using X-ray crystallography or cryo-EM to elucidate mechanistic details of RAB23-mediated membrane trafficking. Current research continues to explore its context-dependent roles in development, cancer, and metabolic disorders.
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