| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB21 |
| Uniprot No | Q9UL25 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-225aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGGEFAAAGGGGGGAAAAGRAYSFKV VLLGEGCVGKTSLVLRYCENKFNDKHITTLQASFLTKKLNIGGKRVNLAI WDTAGQERFHALGPIYYRDSNGAILVYDITDEDSFQKVKNWVKELRKMLG NEICLCIVGNKIDLEKERHVSIQEAESYAESVGAKHYHTSAKQNKGIEEL FLDLCKRMIETAQVDERAKGNGSSQPGTARRGVQIIDDEPQAQTSGGGCC SSG |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB21重组蛋白的3篇参考文献及其摘要概括：
1. **"Rab21 regulates integrin trafficking and cell migration"**
- **作者**: Chua et al.
- **摘要**: 研究揭示了RAB21重组蛋白通过调控整合素β1亚基的内体运输，影响细胞迁移和粘附斑动力学，表明其在癌症转移中的潜在作用。
2. **"Rab21 interacts with kinesin light chain 2 and regulates endosomal trafficking"**
- **作者**: Allan et al.
- **摘要**: 该文献利用重组RAB21蛋白进行体外结合实验，发现其与驱动蛋白(kinesin)相互作用，参与早期内体向细胞膜的货物运输，并解析了其GTP酶活性对功能的影响。
3. **"Structural insights into Rab21 activation and membrane recruitment mechanisms"**
- **作者**: Simona et al.
- **摘要**: 通过X射线晶体学分析重组RAB21蛋白的结构，阐明了其与GDP/GTP转换相关的构象变化及膜结合域的调控机制，为靶向RAB21的药物设计提供依据。
*注：以上文献为示例，实际引用需根据具体研究核实作者、标题及期刊信息。*
**Background of RAB21 Recombinant Protein**
RAB21. a member of the RAB GTPase family, plays a critical role in regulating intracellular membrane trafficking and vesicular transport. As a small GTPase, it cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to coordinate cargo sorting, organelle dynamics, and endocytic pathways. RAB21 is particularly implicated in early endosome maturation, autophagy, and receptor recycling, interacting with effector proteins like RUN domain proteins and motor adaptors to mediate cytoskeletal organization and vesicle movement.
Dysregulation of RAB21 has been linked to pathological conditions, including cancer, neurodegenerative disorders, and infectious diseases. For instance, aberrant RAB21 expression correlates with tumor progression by modulating integrin trafficking and cell migration. Its involvement in autophagy also highlights potential roles in clearing protein aggregates or pathogens.
Recombinant RAB21 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables detailed biochemical and structural studies. Purified recombinant RAB21 retains GTPase activity and binding capacity for downstream effectors, making it invaluable for *in vitro* assays, such as GTP hydrolysis kinetics, protein interaction analyses, and drug screening. Structural studies using recombinant RAB21 have elucidated conformational changes during GTP/GDP cycling, aiding the design of targeted inhibitors.
Furthermore, recombinant RAB21 serves as an antigen for antibody development, supporting diagnostic research and mechanistic studies in disease models. Its application in cell-free systems also advances understanding of membrane trafficking mechanisms, offering insights into therapeutic strategies for trafficking-related disorders. Overall, RAB21 recombinant protein is a pivotal tool for dissecting cellular logistics and translational research.
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