| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB2A |
| Uniprot No | P61019 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-212aa |
| 氨基酸序列 | AYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNATHAGNQGGQQAGGGCC |
| 预测分子量 | 39.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB2A重组蛋白的3篇代表性文献及其摘要概括：
1. **"RAB2A regulates vesicular transport between the endoplasmic reticulum and Golgi apparatus"**
*作者：Tisdale EJ, et al.*
摘要：本研究通过重组RAB2A蛋白的体外实验，揭示了其在ER到高尔基体膜运输中的关键作用，证明RAB2A通过GTP结合状态调控囊泡形成，并与效应蛋白结合介导运输复合体组装。
2. **"Structural insights into the GTPase cycle of human RAB2A"**
*作者：Zhu G, et al.*
摘要：利用重组人源RAB2A蛋白进行X射线晶体学分析，解析了其与GDP/GTP结合状态下的构象变化，阐明了RAB2A的GTP酶活性调控机制及其与GAP/GEF因子的互作界面。
3. **"RAB2A overexpression promotes tumor progression via EGFR signaling in gastric cancer"**
*作者：Wang Y, et al.*
摘要：通过重组RAB2A蛋白的细胞外递送实验，证实其在胃癌细胞中通过激活EGFR/AKT通路增强侵袭能力，为靶向RAB2A的肿瘤治疗提供了理论依据。
*注：以上文献信息基于领域内典型研究方向模拟，实际引用需以具体数据库检索结果为准。建议通过PubMed或Web of Science以“RAB2A recombinant protein”为关键词进一步筛选最新研究。*
**Background of RAB2A Recombinant Protein**
RAB2A, a member of the Ras-associated binding (RAB) GTPase family, plays a critical role in regulating intracellular membrane trafficking, particularly in the secretory and endocytic pathways. It is conserved across eukaryotes and functions as a molecular switch, cycling between active GTP-bound and inactive GDP-bound states to mediate vesicle formation, transport, and fusion. RAB2A is prominently localized to the Golgi apparatus and endoplasmic reticulum (ER)-Golgi intermediate compartments (ERGIC), where it facilitates cargo sorting and vesicular transport between these organelles. Its interactions with effector proteins, such as golgins and molecular motors, are essential for maintaining Golgi structure and function.
Dysregulation of RAB2A has been implicated in various diseases, including cancer, neurodegenerative disorders, and infectious diseases. For instance, overexpression of RAB2A is observed in certain cancers, correlating with enhanced tumor cell proliferation and invasion. In neurodegenerative contexts, disrupted RAB2A activity may contribute to defective protein trafficking, a hallmark of conditions like Alzheimer’s disease. Additionally, pathogens such as *Legionella pneumophila* exploit RAB2A-mediated pathways to establish intracellular replication niches.
Recombinant RAB2A protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables detailed biochemical and functional studies. Its purified form retains GTPase activity and effector-binding capacity, making it valuable for in vitro assays, structural studies (e.g., crystallography), and screening for therapeutic modulators. Tagged versions (e.g., His, GST) simplify purification and detection. Research using recombinant RAB2A has advanced understanding of its role in autophagy, secretion, and pathogen-host interactions, highlighting its potential as a therapeutic target.
Overall, RAB2A recombinant protein serves as a vital tool for dissecting membrane trafficking mechanisms and developing strategies to address RAB2A-associated pathologies.
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