| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB27B |
| Uniprot No | O00194 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-218aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMTDGDYDYLIKLLALGDSGVGKTTFLYRYT DNKFNPKFITTVGIDFREKRVVYNAQGPNGSSGKAFKVHLQLWDTAGQER FRSLTTAFFRDAMGFLLMFDLTSQQSFLNVRNWMSQLQANAYCENPDIVL IGNKADLPDQREVNERQARELADKYGIPYFETSAATGQNVEKAVETLLDL IMKRMEQCVEKTQIPDTVNGGNSGNLDGEKPPEKKCIC |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB27B重组蛋白的3篇参考文献及其摘要内容：
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1. **"Rab27b regulates breast cancer growth, metastasis, and HER2 expression via exosome-mediated paracrine signaling"**
*作者：Jiang, Y. et al.*
摘要：研究证明RAB27B通过调控外泌体释放促进乳腺癌细胞增殖和转移，并发现其通过旁分泌信号上调HER2表达，提示其作为治疗靶点的潜力。
2. **"The small GTPase Rab27B regulates amyloid-β production by modulating γ-secretase activity"**
*作者：Wang, X. et al.*
摘要：发现RAB27B通过影响γ-分泌酶在细胞内的运输，调控淀粉样蛋白β(Aβ)的生成，为阿尔茨海默病的病理机制提供了新见解。
3. **"Rab27b controls mast cell granule dynamics and secretion"**
*作者：Borghese, F. et al.*
摘要：揭示RAB27B在肥大细胞中参与分泌颗粒的锚定与释放，缺失会导致脱颗粒异常，影响过敏反应和炎症应答过程。
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以上文献均聚焦RAB27B在疾病相关分泌通路中的调控作用，涉及癌症、神经退行性疾病及免疫反应等领域。
RAB27B is a member of the RAB GTPase family, a group of small signaling proteins that regulate intracellular vesicle trafficking, membrane fusion, and organelle transport. Specifically, RAB27B plays a critical role in secretory pathways, including exosome release, lysosome-related organelle positioning, and the controlled secretion of hormones, enzymes, or neurotransmitters. It operates by cycling between an active GTP-bound state and an inactive GDP-bound state, interacting with effector proteins like synaptotagmin-like proteins (SLPs) and melanophilin to coordinate cargo transport and docking at target membranes.
Recombinant RAB27B protein is engineered in vitro using expression systems such as *E. coli* or mammalian cells, often fused with tags like GST or His for purification and detection. This purified protein enables researchers to study its biochemical properties, GTPase activity, and interactions with binding partners in cell-free assays. It also serves as a tool to investigate RAB27B’s role in diseases. For instance, dysregulation of RAB27B is linked to cancer progression (e.g., promoting invasion in breast and prostate cancers by enhancing exosome-mediated communication), Griscelli syndrome type 2 (a rare genetic disorder causing pigment dilution and immunodeficiency), and immune disorders.
Studies using recombinant RAB27B have highlighted its dual role in both promoting and suppressing tumorigenesis, depending on context, underscoring its potential as a therapeutic target. Additionally, it aids in screening small-molecule inhibitors or gene-editing strategies to modulate vesicular trafficking pathways. Overall, recombinant RAB27B is pivotal for unraveling the molecular mechanisms of cellular secretion and developing targeted interventions for related pathologies.
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