| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB2B |
| Uniprot No | Q8WUD1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMTYAYLFKYIIIGDTGVGKSCLLLQF TDKRFQPVHDLTIGVEFGARMVNIDGKQIKLQIWDTAGQESFRSITRSYY RGAAGALLVYDITRRETFNHLTSWLEDARQHSSSNMVIMLIGNKSDLESR RDVKREEGEAFAREHGLIFMETSAKTACNVEEAFINTAKEIYRKIQQGLF DVHNEANGIKIGPQQSISTSVGPSASQRNSRDIGSNSGCC |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB2B重组蛋白的假设参考文献示例(注：以下内容为虚构，仅用于演示格式)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant RAB2B in Mammalian Cells*
**作者**：Chen X, et al.
**摘要**：研究报道了在哺乳动物细胞中表达重组RAB2B蛋白的方法，并验证其GTPase活性及在细胞内膜运输中的功能，证明其与特定囊泡标记物的共定位。
2. **文献名称**：*Structural Analysis of Recombinant RAB2B Reveals Key Domains for Effector Binding*
**作者**：Patel R, et al.
**摘要**：通过重组RAB2B蛋白的晶体结构解析，鉴定了其与效应蛋白相互作用的关键结构域，为研究其调控细胞内运输的分子机制提供依据。
3. **文献名称**：*Recombinant RAB2B Purification and Its Role in Autophagosome Formation*
**作者**：Wang L, et al.
**摘要**：利用大肠杆菌系统纯化重组RAB2B，并发现其通过调控自噬相关蛋白(如LC3)的募集，影响自噬体形成过程。
4. **文献名称**：*Development of a High-Throughput Assay for RAB2B GTPase Activity Using Recombinant Protein*
**作者**：Kim H, et al.
**摘要**：基于重组RAB2B蛋白开发了一种荧光检测法，用于高通量筛选调控其GTPase活性的小分子抑制剂，为疾病治疗提供潜在靶点。
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**注**：以上文献为示例，实际引用时请通过学术数据库(如PubMed、Google Scholar)检索真实发表的论文。
RAB2B, a member of the RAB GTPase family, plays a critical role in regulating intracellular membrane trafficking, a process essential for maintaining cellular homeostasis. As a small GTP-binding protein, RAB2B cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to control vesicle formation, transport, and fusion. It is particularly associated with the Golgi apparatus and endoplasmic reticulum (ER), where it facilitates the anterograde transport of secretory vesicles and modulates ER-Golgi intermediate compartment (ERGIC) dynamics. Studies suggest its involvement in autophagy, lysosomal function, and unconventional protein secretion, linking it to cellular stress responses and organelle membrane remodeling.
The recombinant RAB2B protein, produced using genetic engineering techniques (e.g., expression in bacterial or mammalian systems followed by affinity purification), retains the native structure and biochemical properties of the endogenous protein. This allows researchers to study its GTPase activity, interaction with effector molecules (like kinases or tethering complexes), and regulatory mechanisms in vitro. Recombinant forms often include tags (e.g., His, GST) for experimental flexibility in pull-down assays, crystallography, or antibody development.
Dysregulation of RAB2B has been implicated in pathological conditions, including cancer progression, neurodegenerative disorders, and infectious diseases. For example, its overexpression correlates with tumor invasiveness in certain cancers, while mutations may disrupt vesicular transport in neurons, contributing to neurodegeneration. Recombinant RAB2B serves as a tool to investigate these mechanisms, screen therapeutic compounds targeting RAB pathways, or develop diagnostic biomarkers. Ongoing research aims to elucidate its isoform-specific functions and potential as a drug target, emphasizing its emerging significance in cell biology and translational medicine.
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