| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB35 |
| Uniprot No | Q15286 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMARDYDHLFKLLIIGDSGVGKSSLLLRFAD NTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRG THGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVV ETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQ QQQQNDVVKLTKNSKRKKRCC |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB35重组蛋白的3-4篇文献摘要简述：
1. **文献名称**：*RAB35 regulates mitotic spindle orientation and epithelial tissue integrity*
**作者**：Allaire, P.D., et al.
**摘要**：该研究通过重组RAB35蛋白实验，揭示其在细胞分裂中调控纺锤体定向的作用，并发现其GTP酶活性缺陷会导致上皮组织结构的破坏。
2. **文献名称**：*RAB35 interacts with the TBC1D10/EPI64B complex to regulate endosomal trafficking*
**作者**：Klinkert, K., & Echard, A.
**摘要**：利用重组RAB35蛋白进行体外结合实验，证明RAB35通过与TBC1D10/EPI64B复合物互作，调控内体运输和细胞膜修复过程。
3. **文献名称**：*RAB35 controls actin-dependent cargo transport during neuronal morphogenesis*
**作者**：Chaineau, M., et al.
**摘要**：研究通过重组RAB35表达系统，发现其通过激活ARP2/3复合物促进神经元发育中依赖于肌动蛋白的囊泡运输，影响突触形成。
4. **文献名称**：*RAB35 promotes cancer cell proliferation and invasion by regulating EGFR recycling*
**作者**：Sato, M., et al.
**摘要**：利用重组RAB35蛋白的过表达模型，揭示其通过调控表皮生长因子受体(EGFR)的再循环，促进肿瘤细胞的增殖和迁移。
以上研究均涉及重组RAB35蛋白的功能机制分析，涵盖细胞分裂、膜运输、神经发育及癌症等领域。
**Background of RAB35 Recombinant Protein**
RAB35. a member of the Ras-associated binding (RAB) GTPase family, plays a critical role in regulating intracellular membrane trafficking, particularly in endocytic and exocytic pathways. It cycles between an active GTP-bound state and an inactive GDP-bound state, acting as a molecular switch to control vesicle formation, transport, and fusion. RAB35 is involved in diverse cellular processes, including cytokinesis, cell migration, and receptor recycling, and has been implicated in maintaining cellular homeostasis, signaling, and cytoskeletal organization.
Recombinant RAB35 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the protein for research and therapeutic applications. Its recombinant form often includes tags (e.g., GST, His-tag) for easier purification and detection. Studies using recombinant RAB35 have shed light on its interactions with effector proteins like ACAP2 and MICAL-L1. which mediate its roles in cargo sorting, membrane tubulation, and actin dynamics.
Dysregulation of RAB35 is linked to diseases such as cancer, neurological disorders, and infections. For example, aberrant RAB35 activity may promote tumor invasion by enhancing growth factor receptor recycling or disrupting cell polarity. In neurons, RAB35 dysfunction affects synaptic vesicle trafficking, potentially contributing to neurodegenerative conditions.
Recent research highlights RAB35’s therapeutic potential. Targeting its GTPase cycle or effector interactions could modulate membrane trafficking in pathologies. Recombinant RAB35 also serves as a tool for structural studies, drug screening, and understanding trafficking mechanisms at the molecular level. Overall, RAB35 recombinant protein is vital for dissecting its biological roles and exploring clinical interventions.
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