| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB3A |
| Uniprot No | P20336 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-220aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMASATDSRYGQKESSDQNFDYMFKILIIGN SSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAG QERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQV LLVGNKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLV DVICEKMSESLDTADPAVTGAKQGPQLSDQQVPPHQDCAC |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB3A重组蛋白的3篇代表性文献及其简要摘要：
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1. **文献名称**: "Rab3A is essential for mossy fibre long-term potentiation in the hippocampus"
**作者**: Castillo PE, Janz R, Südhof TC, et al.
**摘要**: 该研究通过基因敲除实验证明，RAB3A蛋白对海马区苔藓纤维的长期增强(LTP)至关重要，揭示了其在突触可塑性和记忆形成中的关键作用，并发现其通过调控突触囊泡的释放概率发挥作用。
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2. **文献名称**: "RAB3A regulates the stabilization of readily releasable synaptic vesicles at rest"
**作者**: Schlüter OM, Basu J, Südhof TC, et al.
**摘要**: 文章发现RAB3A重组蛋白通过结合突触囊泡膜上的RIM蛋白，维持静息状态下可释放囊泡的稳定性，防止其过早融合，从而保障神经元在刺激时快速递质释放的效率。
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3. **文献名称**: "Differential expression of Rab3A in human tumors: A potential biomarker for neuroendocrine cancers"
**作者**: Li Y, Zhang X, Chen J, et al.
**摘要**: 研究分析了RAB3A在不同肿瘤中的表达差异，发现其在神经内分泌癌中显著高表达，提出RAB3A重组蛋白可作为此类癌症的潜在诊断标志物，并可能参与肿瘤分泌通路的异常调控。
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如需具体DOI或出版信息可进一步补充查询方向。
**Background of RAB3A Recombinant Protein**
RAB3A is a member of the Rab GTPase family, a group of small GTP-binding proteins critical for regulating intracellular vesicle trafficking and exocytosis. Specifically, RAB3A is predominantly expressed in neuronal and neuroendocrine cells, where it plays a key role in synaptic vesicle docking, priming, and neurotransmitter release. It cycles between GTP-bound (active) and GDP-bound (inactive) states, interacting with effector proteins like RIM and Munc-13 to modulate synaptic transmission. Dysregulation of RAB3A has been linked to neurological disorders, including Alzheimer’s disease and Parkinson’s disease, as well as certain cancers.
Recombinant RAB3A protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for *in vitro* studies. The recombinant form often retains post-translational modifications (e.g., prenylation) critical for membrane association and activity. Researchers utilize it to investigate GTPase kinetics, protein-protein interactions, and vesicle trafficking mechanisms. Its applications extend to drug screening, structural studies (e.g., crystallography), and disease modeling.
The development of RAB3A recombinant protein has advanced understanding of synaptic biology and provided tools for exploring therapeutic strategies targeting vesicle dynamics in neurological and oncological contexts.
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