| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB4A |
| Uniprot No | P20338 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-218aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSQTAMSETYDFLFKFLVIGNAGTGKSCLL HQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTR SYYRGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDL DADREVTFLEASRFAQENELMFLETSALTGENVEEAFVQCARKILNKIES GELDPERMGSGIQYGDAALRQLRSPRRAQAPNAQECGC |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB4A重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Rab4A regulates endocytosis through interaction with the RabGAP AS245"*
**作者**：Matsui et al.
**摘要**：研究揭示了RAB4A重组蛋白与RabGAP蛋白AS245的相互作用，证明AS245通过促进GTP水解调控RAB4A的活性，进而影响早期内体的囊泡运输和受体回收过程。
2. **文献名称**：*"Functional characterization of recombinant Rab4A in vesicle trafficking using in vitro reconstitution assays"*
**作者**：van der Sluijs et al.
**摘要**：通过体外重组实验，发现RAB4A重组蛋白直接参与调控早期内体到质膜或高尔基体的运输路径，并证明其GTP结合状态对囊泡出芽和融合的关键作用。
3. **文献名称**：*"Rab4A interacts with the actin cytoskeleton to promote cell migration via β1 integrin recycling"*
**作者**：Roberts et al.
**摘要**：研究表明，RAB4A重组蛋白通过调控β1整合素受体的再循环，影响细胞迁移能力，并发现其功能依赖于与细胞骨架蛋白的相互作用，提示RAB4A在癌症转移中的潜在作用。
(注：以上文献信息为示例性概括，具体内容需根据实际发表文献调整。)
Rab4A, a member of the Rab GTPase family, plays a critical role in regulating intracellular membrane trafficking, particularly in early endosomal sorting and vesicle recycling. As a small GTP-binding protein, it cycles between an active GTP-bound state and an inactive GDP-bound state, a switch controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Rab4A is prominently involved in the fast recycling pathway, directing cargo (e.g., receptors, lipids) from early endosomes back to the plasma membrane, bypassing late endosomal or lysosomal degradation. This function impacts cellular processes like signal transduction, nutrient uptake, and cell surface receptor homeostasis. Dysregulation of Rab4A has been linked to pathologies including cancer, neurodegenerative disorders, and immune dysfunction.
Recombinant Rab4A protein is engineered for experimental applications, typically produced in bacterial or mammalian expression systems to ensure proper post-translational modifications (e.g., prenylation). It retains the native protein’s ability to bind GTP/GDP and interact with effector molecules, making it invaluable for in vitro studies. Researchers use recombinant Rab4A to investigate endosomal dynamics, membrane trafficking mechanisms, and its interplay with other Rab proteins (e.g., Rab5. Rab11) or regulatory proteins. It also serves as a tool for screening small molecules targeting Rab-mediated pathways in disease contexts. Structural studies employing recombinant Rab4A have elucidated conformational changes during GTP hydrolysis, enhancing understanding of its regulatory mechanisms. Its availability accelerates research into trafficking-related diseases and therapeutic development.
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