| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB5C |
| Uniprot No | P51148 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAGRGGAARPNGPAAGNKICQFKLVLL GESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTVKFEIWDT AGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASPNI VIALAGNKADLASKRAVEFQEAQAYADDNSLLFMETSAKTAMNVNEIFMA IAKKLPKNEPQNATGAPGRNRGVDLQENNPASRSQCCSN |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB5C重组蛋白的参考文献及其摘要概括(内容基于领域内典型研究方向虚构，仅供参考)：
1. **《RAB5C调控内吞体成熟的分子机制研究》**
作者：Zhang et al.
摘要：通过表达并纯化RAB5C重组蛋白，研究其与效应蛋白(如EEA1)的相互作用，揭示RAB5C通过GTP水解调控早期内吞体融合的分子机制。
2. **《RAB5C在乳腺癌细胞迁移中的功能解析》**
作者：Wang et al.
摘要：利用重组RAB5C蛋白进行体外功能实验，证明其过表达通过激活EGFR信号通路促进乳腺癌细胞迁移，提示其作为潜在治疗靶点。
3. **《RAB5C重组蛋白的晶体结构及其GTP结合特性》**
作者：Li et al.
摘要：首次解析RAB5C重组蛋白的晶体结构，揭示其GTP结合口袋的构象变化，为设计特异性抑制剂提供结构基础。
4. **《RAB5C与自噬相关蛋白LC3的相互作用研究》**
作者：Chen et al.
摘要：通过重组蛋白共沉淀实验，发现RAB5C与LC3在营养缺失条件下存在直接结合，表明其参与调控自噬体形成的交叉调控机制。
注：以上文献名为虚构，仅反映该领域常见研究方向，实际文献需通过数据库(如PubMed、Web of Science)检索确认。
**Background of RAB5C Recombinant Protein**
RAB5C is a member of the RAB GTPase family, a group of small GTP-binding proteins essential for regulating intracellular vesicle trafficking and membrane dynamics. Specifically, RAB5C localizes to early endosomes and plays a pivotal role in clathrin-mediated endocytosis, endosomal sorting, and early-stage vesicle fusion. It cycles between an active GTP-bound state and an inactive GDP-bound state, interacting with effector proteins like EEA1 and Rabaptin-5 to mediate membrane tethering, cargo sorting, and transport. Dysregulation of RAB5C has been linked to pathologies such as cancer, neurodegenerative disorders, and immune dysfunction, highlighting its importance in cellular homeostasis.
Recombinant RAB5C protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to produce a purified, functional form of the protein. This allows researchers to study its biochemical properties, GTPase activity, and interactions in controlled settings. The recombinant protein often includes tags (e.g., His or GST) for simplified purification and detection. Applications span mechanistic studies (e.g., mutations affecting GTP hydrolysis), drug screening for RAB5C-targeted therapies, and diagnostic assays as a reference control.
By enabling precise analysis of RAB5C’s role in vesicle transport and disease pathways, recombinant RAB5C serves as a critical tool for advancing molecular cell biology and therapeutic development.
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