| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABIF |
| Uniprot No | P47224 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-123aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MEPAEQPSELVSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV SHE |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RABIF重组蛋白的示例参考文献(注：文献为示例性内容，非真实发表论文)：
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1. **文献名称**: "Functional characterization of recombinant RABIF protein in vesicular trafficking"
**作者**: Smith A, et al.
**摘要**: 研究通过大肠杆菌系统表达重组RABIF蛋白，验证其作为Rab GTP酶效应蛋白的功能，证明其在细胞内囊泡运输中调控Rab9蛋白的活性。
2. **文献名称**: "Expression and purification of human RABIF for structural analysis"
**作者**: Chen L, Wang Y.
**摘要**: 描述利用昆虫细胞表达系统高效制备人源重组RABIF蛋白，并通过X射线晶体学解析其与Rab3A结合的复合物结构，揭示其分子互作机制。
3. **文献名称**: "RABIF recombinant protein enhances lysosomal degradation in cellular models"
**作者**: Kim S, et al.
**摘要**: 利用哺乳动物细胞表达的重组RABIF蛋白，发现其通过激活Rab7通路促进自噬体-溶酶体融合，为溶酶体功能缺陷疾病提供潜在治疗策略。
4. **文献名称**: "Role of RABIF in Rab protein prenylation: Insights from in vitro reconstitution assays"
**作者**: Gonzalez E, et al.
**摘要**: 通过体外重组实验证明RABIF作为分子伴侣协助Rab蛋白的异戊二烯化修饰，并开发基于重组RABIF的高通量药物筛选平台。
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注：以上文献为模拟内容，实际引用时需检索PubMed、Web of Science等数据库获取真实文献。
**Background of RABIF Recombinant Protein**
RABIF (RAB interacting factor), also known as MSS4 or RABGEF1. is a conserved regulatory protein involved in intracellular vesicle trafficking and membrane dynamics. It functions as a guanine nucleotide exchange factor (GEF) for RAB GTPases, a subfamily of small GTP-binding proteins that orchestrate vesicle formation, transport, tethering, and fusion. Specifically, RABIF facilitates the activation of RAB proteins by promoting the exchange of GDP for GTP, a critical step in their functional cycling between inactive (GDP-bound) and active (GTP-bound) states. This activity positions RABIF as a key modulator of endocytic and secretory pathways, impacting cellular processes such as autophagy, receptor recycling, and neurotransmitter release.
The recombinant RABIF protein is engineered using biotechnological platforms (e.g., bacterial or mammalian expression systems) to produce purified, functional forms of the protein for research and therapeutic applications. Its recombinant version retains the ability to interact with and activate target RAB proteins, making it a valuable tool for studying RAB-mediated signaling networks, membrane trafficking defects, and diseases linked to dysregulated vesicle transport, such as cancer, neurodegenerative disorders, and immune dysfunctions.
Research on RABIF also explores its role in cellular homeostasis and disease mechanisms. For instance, its interaction with RAB8 and RAB13 highlights its involvement in polarized secretion and cell migration, relevant to cancer metastasis. Additionally, RABIF’s conserved structure across species underscores its fundamental biological importance. By leveraging recombinant RABIF, scientists aim to dissect molecular pathways, screen for modulators of RAB activity, and develop targeted therapies for trafficking-related disorders. This protein thus bridges basic cell biology and translational applications in biomedicine.
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