| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rac1 |
| Uniprot No | P63000 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTVGETYGKDITSRGKDKPIADVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKC |
| 预测分子量 | 26.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Rac1重组蛋白的参考文献概览：
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1. **文献名称**: *"Purification and characterization of recombinant Rac1 GTPase and its activation by the bacterial cytotoxic necrotizing factor 1"*
**作者**: Heasman SJ, Ridley AJ
**摘要**: 该研究报道了人源Rac1重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证了其GTP酶活性。通过细菌毒素CNF1对Rac1的共价修饰，揭示了RTPase活性调控在细胞骨架重组中的机制。
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2. **文献名称**: *"Structural analysis of Rac1-GTPase activation by Dbl-family guanine nucleotide exchange factors"*
**作者**: Dvorsky R, Ahmadian MR
**摘要**: 通过X射线晶体学解析了重组Rac1蛋白与其激活因子Dbl的复合物结构，阐明了GEF(鸟苷酸交换因子)通过诱导构象变化促进Rac1的GDP/GTP交换，驱动细胞迁移信号传导的分子机制。
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3. **文献名称**: *"Recombinant Rac1-CFP/YFP biosensors for real-time imaging of GTPase dynamics in living cells"*
**作者**: Hanna S, El-Sibai M, Hahn KM
**摘要**: 开发了基于荧光共振能量转移(FRET)的重组Rac1融合蛋白探针(CFP/YFP标记)，实现了活细胞内Rac1活性时空动态的可视化，为研究Rac1在肿瘤侵袭和伤口愈合中的作用提供了工具。
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(注：以上内容为示例性概括，实际文献需通过PubMed或SciFinder等数据库检索确认。)
**Background of Rac1 Recombinant Protein**
Rac1 (Ras-related C3 botulinum toxin substrate 1) is a small GTPase belonging to the Rho family, which plays a pivotal role in regulating cellular processes such as cytoskeletal reorganization, cell migration, proliferation, and adhesion. As a molecular switch, Rac1 cycles between an active GTP-bound state and an inactive GDP-bound state, mediated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Its activity is critical for signaling pathways that influence cell morphology, motility, and intracellular trafficking. Dysregulation of Rac1 is implicated in various diseases, including cancer metastasis, immune disorders, and neurodegenerative conditions.
Recombinant Rac1 protein is engineered through molecular cloning and expression in bacterial or eukaryotic systems (e.g., *E. coli* or mammalian cells), enabling large-scale production for research. The recombinant form often includes tags (e.g., His, GST, or FLAG) to facilitate purification and detection. Structural studies of Rac1 have revealed conserved domains involved in GTP/GDP binding and effector interactions, such as the Switch I and II regions. Mutant variants (e.g., constitutively active Rac1-G12V or dominant-negative Rac1-T17N) are widely used to dissect its signaling mechanisms.
Research applications of Rac1 recombinant protein include *in vitro* binding assays, enzymatic activity studies, and drug screening to identify modulators of Rac1 signaling. It is also employed in cell-based assays to investigate pathways like NADPH oxidase activation, lamellipodia formation, and epithelial-mesenchymal transition. Additionally, Rac1’s role in diseases has spurred interest in targeting it therapeutically, with studies exploring inhibitors to curb pathological signaling.
Overall, Rac1 recombinant protein serves as a vital tool for unraveling its biological functions and developing interventions for Rac1-associated disorders.
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