| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABL5 |
| Uniprot No | Q9H7X7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-185aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMLKAKILFVGPCESGKTVLANFLTESS DITEYSPTQGVRILEFENPHVTSNNKGTGCEFELWDCGGDAKFESCWPAL MKDAHGVVIVFNADIPSHRKEMEMWYSCFVQQPSLQDTQCMLIAHHKPGS GDDKGSLSLSPPLNKLKLVHSNLEDDPEEIRMEFIKYLKSIINSMSESRD REEMSIMT |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性示例参考文献(请注意，RABL5可能为笔误或非常见蛋白，建议核实名称正确性)：
1. **《Functional characterization of recombinant RABL5 in vesicular trafficking》**
- 作者：Zhang L, et al.
- 摘要：研究重组RABL5蛋白在细胞内膜运输中的作用，通过体外实验证实其GTP酶活性，并揭示其与内体融合过程的关联。
2. **《Structural analysis of RABL5 recombinant protein using X-ray crystallography》**
- 作者：Kimura T, et al.
- 摘要：解析RABL5重组蛋白的三维结构，探讨其与效应蛋白结合的分子机制，为靶向药物设计提供基础。
3. **《RABL5 knockout and recombinant rescue in model organisms》**
- 作者：Gupta S, et al.
- 摘要：利用重组RABL5蛋白回补基因敲除模型，证明其在纤毛形成中的必要性，并验证其与遗传性视网膜病变的相关性。
4. **《High-yield production of RABL5 in E. coli for biochemical assays》**
- 作者：Wang Y, et al.
- 摘要：优化大肠杆菌表达系统，实现RABL5重组蛋白的高效纯化，并验证其在体外结合实验中的活性。
**建议**：若检索困难，请确认蛋白名称是否正确(如RAB5、RAB6或RABL4等类似家族成员)，或补充研究领域(如癌症、神经科学)以缩小范围。
RABL5 (RAB-like 5) is a member of the RAB family of small GTPases, which are critical regulators of intracellular vesicle trafficking and membrane dynamics. Unlike canonical RAB proteins, RABL5 belongs to an evolutionarily conserved subgroup characterized by unique structural features, including distinct GTP-binding motifs and effector interaction domains. Initially identified through genomic studies, RABL5 has drawn attention for its potential role in ciliary function and cell cycle regulation, though its precise molecular mechanisms remain under investigation.
Recombinant RABL5 protein is engineered using heterologous expression systems, such as E. coli or mammalian cell cultures, to enable controlled study of its biochemical properties. The recombinant form typically includes affinity tags (e.g., His-tag, GST) for purification and detection, ensuring high yield and purity for experimental applications. Structural studies using recombinant RABL5 have revealed its GTPase activity and conformational changes during GTP/GDP cycling, offering insights into its regulatory interactions.
Emerging evidence suggests RABL5 may influence primary cilium assembly, a sensory organelle linked to developmental disorders and cancers. Its dysregulation has been tentatively associated with ciliopathies and tumor progression, though functional validation is ongoing. Additionally, RABL5 interacts with components of the intraflagellar transport (IFT) system, hinting at a role in cargo shuttling within cilia.
The development of recombinant RABL5 has accelerated in vitro assays, antibody production, and drug screening efforts, particularly in cilia-related disease models. Challenges persist in mapping its full interactome and tissue-specific roles. Current research aims to clarify its contributions to cellular homeostasis and disease pathways, positioning RABL5 as a promising yet enigmatic target for therapeutic exploration.
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