| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD1 |
| Uniprot No | O60671 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPLLTQQIQDEDDQYSLVASLDNVRNLSTI LKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESV TFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVT VCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQIT MSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKP STKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPES ES |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAD1重组蛋白的3篇参考文献概览(注：文献为示例性内容，实际引用时需核实准确性)：
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1. **文献名称**: *"Recombinant human RAD1 protein: purification and functional characterization in nucleotide excision repair"*
**作者**: Smith A, et al.
**摘要**: 研究报道了人源RAD1重组蛋白的表达与纯化方法，并验证其与RAD10形成复合物后，在体外核苷酸切除修复(NER)中识别DNA损伤的活性，强调了RAD1在修复紫外诱导损伤中的关键作用。
2. **文献名称**: *"Structural analysis of the RAD1-RAD10 endonuclease complex in DNA damage checkpoint signaling"*
**作者**: Jones B, et al.
**摘要**: 通过X射线晶体学解析RAD1-RAD10重组复合物的三维结构，揭示了其在DNA损伤检查点调控中的构象变化，并阐明其对异常DNA结构(如单链缺口)的特异性切割机制。
3. **文献名称**: *"RAD1 interacts with Hus1 to form a checkpoint clamp involved in genomic stability"*
**作者**: Lee C, et al.
**摘要**: 研究利用重组RAD1蛋白证明其与Hus1、Rad9形成异源三聚体(9-1-1复合物)，在DNA复制压力下被招募到染色质，通过激活ATR激酶通路维持基因组稳定性。
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如需具体文献，建议在PubMed或Google Scholar中以关键词“RAD1 recombinant”、“RAD1-RAD10 complex”或“9-1-1 clamp”检索近年研究。
**Background of RAD1 Recombinant Protein**
RAD1. a critical protein in DNA damage response and repair, is a member of the RAD1/Hus1 family, forming the evolutionarily conserved 9-1-1 complex (RAD9-RAD1-HUS1) with HUS1 and RAD9. This ring-shaped complex structurally resembles the proliferating cell nuclear antigen (PCNA) clamp and plays a pivotal role in cell cycle checkpoint signaling and DNA damage tolerance. RAD1 is essential for maintaining genomic stability by detecting DNA lesions, stalled replication forks, or abnormal DNA structures, thereby activating the ATR (ataxia-telangiectasia and Rad3-related)-mediated checkpoint pathway to halt the cell cycle and facilitate repair.
Recombinant RAD1 protein is produced via genetic engineering in heterologous systems like *E. coli* or mammalian cells, enabling high-purity studies of its biochemical functions and interactions. Its production often includes tags (e.g., His-tag) for efficient purification. Researchers utilize RAD1 recombinant protein to investigate its role in DNA repair mechanisms, checkpoint regulation, and replication stress responses. Structural studies reveal how RAD1 binds DNA or partners like RAD9 and HUS1. while functional assays explore its interplay with ATR-ATRIP, Claspin, and TOPBP1 in damage signaling.
Dysregulation of RAD1 is linked to cancer, aging, and genetic disorders due to impaired DNA repair. Recombinant RAD1 aids in drug discovery targeting DNA repair pathways, offering potential therapeutic strategies for cancers with defective checkpoint mechanisms. Additionally, it serves as a tool to study mutagenesis, replication fidelity, and responses to chemotherapeutic agents. Understanding RAD1's molecular mechanisms through recombinant protein studies enhances insights into genome maintenance and disease pathogenesis.
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