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产品描述：人FAM20A蛋白(FAM20A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FAM20A含量。FAM20A是一种可能被分泌并可能在造血功能中发挥作用的蛋白质，其突变与釉质发育不全和牙龈增生综合征有关。FAM20A在分泌蛋白的磷酸化过程中起着至关重要的作用，特别是那些细胞外结构域中含有丝氨酸（Ser）或苏氨酸（Thr）残基的蛋白。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于细胞生物学、遗传性疾病模型等领域的蛋白表达调控研究，可为探究FAM20A在矿化组织发育、病理性钙化机制及遗传性疾病分子通路中的功能提供可靠工具，满足科研实验的高通量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FAM20A ELISA kit; UNQ9388/PRO34279Pseudokinase FAM20A ELISA kit
缩写：FAM20A
Uniprot No.：Q96MK3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FAM20A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 82-91 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-107 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of human FAM20A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-89 | ||||
| EDTA plasma (n=4) | 100 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5782.4722.5252.43251.6941.6761.6851.5922.51.0931.0631.0780.9851.250.5640.5190.5420.4490.6250.2670.2830.2750.1820.3120.1970.1880.1930.1000.1560.1120.1090.1110.01800.0960.0890.093 | ||||||
货期：3-5 working days
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